Resazurin sodium salt

CHF 66.00
In stock
CDX-R0051-G0055 gCHF 66.00
CDX-R0051-G02020 gCHF 203.00
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Product Details
Synonyms Alamar Blue; Vybrant; UptiBlue; 7-Hydroxy-3H-phenoxazin-3-one-10-oxide sodium salt
Product Type Chemical
Properties
Formula

C12H6NNaO4

MW 251.17
CAS 62758-13-8
RTECS SP7700000
Source/Host Chemicals Synthetic.
Purity Chemicals ~80% (Dye Content) (UV/Vis). Suitable for cell culture application.
Appearance Black-Violet solid.
Solubility Soluble in water (1mg/ml).
Identity Determined by 1H-NMR.
Declaration Manufactured by Chemodex.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

InChi Key IVGPGQSSDLDOLH-UHFFFAOYSA-M
Smiles [Na+].[O-]C1=CC=C2C(OC3=CC(=O)C=CC3=[N+]2[O-])=C1
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at +4°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Resazurin (also known as Alamar Blue), is the N-oxide of the fluorescent dye resorufin. It is useful for detecting reductive activities in cells and has been widely used for measuring cell proliferation and mitochondrial metabolic activity. It is used as a quantifiable detection agent for enzyme activity assays and as an oxidation-reduction indicator in cell proliferation, cell viability and cytotoxicity assays.

Resazurin is a non-toxic, water-soluble and redox-sensitive dye that is non-fluorescent until it is reduced to the highly red fluorescent resorufin (λEx/λEm: 563/587 nm). Usually, NADPH or NADH is the reductant that converts resazurin to resorufin in the presence of diaphorase as the enzyme. Thus, resazurin can be used to detect NADH, NADPH, or diaphorase levels. Furthermore, the resazurin/diaphorase/NADPH system can be used to detect any biochemical or enzyme activity that is involved in a biochemical reaction generating NADH or NADPH.

Product References

(1) J.  O'Brien, et al.; Eur. J. Bioch. 267, 5421 (2000) | (2) D.P. Penney, et al.; Biotech. Histochem. 77, 237 (2002) | (3) S.N. Rampersad; Sensors 12, 12347 (2012) | (4) R.W. Sabnis; Handbook of Fluorescent Dyes and Probes 370 (2015) | (5) M. Koyanagi, et al.; Cytotechnology 68, 1489 (2016)

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