|Synonyms||3,3′,5,5′-Tetramethylbenzidine dihydrochloride; TMBZ|
C16H20N2 . 2HCl
|MW||240.3 . 72.9|
|Purity Chemicals||≥98% (Titration)|
|Appearance||White to off-white to pink powder.|
|Solubility||Soluble in DMSO.|
|Identity||Determined by NMR.|
|Declaration||Manufactured by Chemodex.|
|Other Product Data||
Click here for Original Manufacturer Product Datasheet
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||+4°C|
Keep cool and dry.
Protect from light and moisture.
|Use/Stability||Stable for at least 2 years after receipt when stored at +4°C.|
|Product Specification Sheet|
TMBZ is a chromogenic reagent utilized for peroxidase detection. It has been developed as an alternative to benzidine. Although the TMBZ solution is colorless, it turns bluish-green (λmax: 655 nm) in the presence of hydrogen peroxide and peroxidase. The structure of this bluish-green complex is thought to be a radical form of two oxidized TMBZ molecules. This peroxidase substrate is used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2M H2SO4 (resulting in a yellow color), and read at 450nm.
(1) V.R. Holland, et al.; Tetrahedron 30, 3299 (1974) | (2) P.E. Thomas, et al.; Anal. Biochem. 75, 168 (1976) | (3) H.H. Liem, et al.; Anal. Biochem. 98, 388 (1979) | (4) E.S. Bos, et al.; J. Immunoassay 2, 187 (1981) | (5) K. Suzuki, et al.; Anal. Biochem. 132, 345 (1983) | (6) F.H. Pujol, et al.; J. Immunoassay. 14, 21 (1993) | (7) F.B. Serrat; Talanta 41, 2091 (1994)