TMB dihydrochloride

CHF 61.00
In stock
CDX-T0151-G0011 gCHF 61.00
CDX-T0151-G0055 gCHF 244.00
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Product Details
Synonyms 3,3′,5,5′-Tetramethylbenzidine dihydrochloride; TMBZ
Product Type Chemical

C16H20N2 . 2HCl

MW 240.3 . 72.9
CAS 64285-73-0
Source/Host Chemicals Synthetic
Purity Chemicals ≥95% (HPLC)
Appearance White to off-white to pink powder.
Solubility Soluble in DMSO.
Identity Determined by NMR.
Declaration Manufactured by Chemodex.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

Smiles Cl[H].Cl[H].CC1=CC(=CC(C)=C1N)C1=CC(C)=C(N)C(C)=C1
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice Keep cool and dry.
Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at +4°C.
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

TMBZ is a chromogenic reagent utilized for peroxidase detection. It has been developed as an alternative to benzidine. Although the TMBZ solution is colorless, it turns bluish-green (λmax: 655 nm) in the presence of hydrogen peroxide and peroxidase. The structure of this bluish-green complex is thought to be a radical form of two oxidized TMBZ molecules. This peroxidase substrate is used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2M H2SO4 (resulting in a yellow color), and read at 450nm.

Product References

(1) V.R. Holland, et al.; Tetrahedron 30, 3299 (1974) | (2) P.E. Thomas, et al.; Anal. Biochem. 75, 168 (1976) | (3) H.H. Liem, et al.; Anal. Biochem. 98, 388 (1979) | (4) E.S. Bos, et al.; J. Immunoassay 2, 187 (1981) | (5) K. Suzuki, et al.; Anal. Biochem. 132, 345 (1983) | (6) F.H. Pujol, et al.; J. Immunoassay. 14, 21 (1993) | (7) F.B. Serrat; Talanta 41, 2091 (1994)

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