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Chemodex
Thiazolyl blue tetrazolium bromide [MTT]
61
CHF
CHF 61.00
In stock
CDX-T0186-G0011 gCHF 61.00
CDX-T0186-G0055 gCHF 244.00
CDX-T0186-G01010 gCHF 434.00
| Product Details | |
|---|---|
| Synonyms | 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide; MTT; Methylthiazolyldiphenyl-tetrazolium bromide; NSC 60102 |
| Product Type | Chemical |
| Properties | |
| Formula | C18H16BrN5S |
| MW | 414.32 |
| CAS | 298-93-1 |
| RTECS | XF8060000 |
| Source/Host Chemicals | Synthetic |
| Purity Chemicals | ≥98% (HPLC) |
| Appearance | Yellow powder. |
| Solubility | Soluble in water (5mg/ml). |
| Identity | Determined by 1H-NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | AZKSAVLVSZKNRD-UHFFFAOYSA-M |
| Smiles | CC1=C(C)SC([N+]2=NC(C3=CC=CC=C3)=NN2C4=CC=CC=C4)=N1.[Br-] |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice | Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at +4°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
MTT is a common histochemical/cytochemical reagent and for the detection of NAD. MTT is used to detect reductive metabolism in cells. It is a cell-permeable yellow dye that is reduced by intracellular NAD(P)H-oxidoreductases in mitochondria of living cells to form the dark blue water-insoluble MTT-formazan. The intensity of the color can then be measured spectrophotometrically at 540 or 570nm, which allows quantitation of cell viability and cell proliferation. It is frequently used in colorimetric assays to measure cell proliferation/cell growth, cytotoxicity (such as for screening cancer drugs), and apoptosis.
Product References
(1) M.A. Abdallah, & J.-F. Biellmann; Eur. J. Biochem. 112, 331 (1980) | (2) Y. Liu, et al.; J. Neurochem. 69, 581 (1997) | (3) M.V. Berridge, et al.; Biotechnol. Ann. Rev. 11, 127 (2005) | (4) T. Hayon, et al.; Leuk. Lymphoma 44, 1957 (2003) (Review) | (5) K. Praebst, et al.; Methods Mol. Biol. 1601, 1 (2017) | (6) P. Kumar, et al.; Cold Spring Harb. Protoc. 2018, pdb.prot095505 (2018) | (7) E. Grela, et al.; Acta Histochem. 120, 303 (2018) (Review)