IL-7 (human) (rec.) (His)

CHF 240.00
In stock
CHI-HF-20107-C01010 µgCHF 240.00
CHI-HF-20107-C05050 µgCHF 710.00
 
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Product Details
Synonyms Interleukin-7; Lymphopoietin 1; LP-1
Product Type Protein
Properties
Source/Host HEK 293 cells
Sequence The extracellular domain of human IL-7 (aa 26-177) is fused at the C-terminus to a His-tag.
Crossreactivity Human
Biological Activity Measured by the dose dependent stimulation of the proliferation of murine 2E8 cells. The ED50 for this effect is typically 0.2ng/ml corresponding to a specific activity of 5x 106 units/mg.
MW ~27kDa (SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.01EU/μg protein (LAL test; Lonza).
Reconstitution Reconstitute 10µg vial with 100 µl sterile water to a concentration of 0.1mg/ml.
Reconstitute 50µg vial with 100 µl sterile water to a concentration of 0.5mg/ml.
Add 1X PBS to the desired protein concentration.
Formulation Lyophilized from 0.2μm-filtered solution in PBS.
Other Product Data NCBI reference AAH47698.1: IL-7 (human)
Declaration Manufactured by Chimerigen.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF
Interleukin-7 (IL-7), is a hematopoietic growth factor and a member of the IL-7/IL-9 family. IL-7 stimulates the proliferation of lymphoid progenitors and is secreted by stromal cells in the bone marrow and thymus. IL-7 is also important for proliferation during certain stages of B cell maturation. IL-7 and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. It is found to be a cofactor for V(D)J rearrangement of the T cell receptor β (TCRβ) during early T cell development. IL-7 can be produced locally by intestinal epithelial and epithelial goblet cells and may serve as a regulatory factor for intestinal mucosal lymphocytes.
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