IL-8 (human) (rec.) (His)
|Synonyms||Interleukin-8; CXCL8; C-X-C Motif Chemokine 8; Emoctakin; GCP-1; LAI; MDNCF; MONAP; NAP-1; NCF; TCF|
|Sequence||Human IL-8 (aa 28-99) is fused at the C-terminus to a His-tag.|
|Endotoxin Content||<0.1EU/μg protein (LAL test; Lonza).|
|Reconstitution||Reconstituted in sterile H2O not less than 100μg/ml, which can then be further diluted in other aqueous solutions.|
|Formulation||Lyophilized from 0.2μm-filtered solution in PBS.|
|Other Product Data||NCBI reference NP_000575.1: IL-8 (human)|
|Declaration||Manufactured by Chimerigen.|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
|Product Specification Sheet|
Interleukin-8 (IL-8) was originally discovered as a neutrophil chemotactic and activating factor and is a member of the α (CXC) subfamily of chemokines (including also platelet factor 4, GRO, IP-10, etc.). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes and various tumor cell lines, produce IL-8 in response to a wide variety of proinflammatory stimuli such as exposure to IL-1, TNF, LPS and viruses. IL-8 has a wide range of other proinflammatory effects. It is a potent chemoattractant for neutrophils and causes degranulation of neutrophil specific granules and azurophilic granules. IL-8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and subendothelial matrix proteins. Besides neutrophils, IL-8 is also chemotactic for basophils, T cells and eosinophils. IL-8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. IL-8 was also reported to be angiogenic both in vivo and in vitro.