IL-18 (human) (rec.)

CHF 180.00
In stock
CHI-HR-20618-C01010 µgCHF 180.00
CHI-HR-20618-C05050 µgCHF 540.00
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Product Details
Synonyms Interleukin-18; Interferon-γ-inducing Factor; IGIF; IL-1γ; IL1F4
Product Type Protein
Properties
Source/Host E. coli
Sequence

Human IL-18 (aa 37-193) is untagged.

Crossreactivity Human
MW ~18kDa
Purity ≥98% (SDS-PAGE)
Endotoxin Content <0.06EU/μg purified protein (LAL test; Lonza).
Reconstitution Reconstituted in sterile H2O not less than 100μg/ml, which can then be further diluted in other aqueous solutions.
Formulation Lyophilized from 0.2μm-filtered solution in 1X PBS.
Other Product Data

NCBI reference NP_001553.1: IL-18 (human)

Declaration Manufactured by Chimerigen.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

Interleukin-18 (IL-18) is a costimulatory factor for production of interferon-γ (IFN-γ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE; Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-γ is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-γ activates macrophages and enhances NK activity and B cell maturation, proliferation and Ig secretion. IFN-γ also induces expression of MHC class I and II antigens and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-γ. Pleiotropic effects of IL-18 have also been reported, such as enhancement production of IFN-γ and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF, IFN-γ in T cells and enhancement of Fas ligand expression by Th1 cells.

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