CD152 [CTLA-4] (mouse):Fc (mouse) (rec.)

CHF 140.00
In stock
CHI-MF-110A4-C100100 µgCHF 140.00
CHI-MF-110A4-C500500 µgCHF 410.00
More Information
Product Details
Synonyms CTLA-4
Product Type Protein
Properties
Source/Host NS1 cells
Sequence

The extracellular domain of mouse CD152 [CTLA-4] (aa 38-160) is fused to the N-terminus of the Fc region of mouse IgG2a.

Crossreactivity Mouse
Biological Activity

Binds both CD80 (B7-1) and CD86 (B7-2) with high affinity and inhibits CD28 signaling competitively. Kills the target cell completely.

Purity ≥98% (SDS-PAGE)
Endotoxin Content <0.06EU/μg protein (LAL test; Lonza).
Reconstitution Reconstitute at 1mg/ml in sterile water.
Add 1X PBS to the desired protein concentration.
Formulation Lyophilized from 0.2μm-filtered solution in PBS.
Protein Negative Control

Fc (mouse) IgG2a Control (rec.)

Other Product Data

NCBI reference NP_033973.2: CD152 (mouse)

Declaration Manufactured by Chimerigen.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

CD152 [CTLA-4] and CD28, together with their ligands B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD152 and CD28 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CD152 and CD28 are composed of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. CD152 and CD28 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. CD152 was originally identified as a gene that was specifically expressed by cytotoxic T lymphocytes. However, CD152 transcripts have since been found in both Th1 and Th2, and CD4+ and CD8+ T cell clones. Whereas, CD28 expression is constitutive on the surfaces of 95% of CD4+ T cells and 50% of CD8+ T cells and is down regulated upon T cell activation, CD152 expression is upregulated rapidly following T cell activation and peaks approximately 24 hours following activation. Although both CD152 and CD28 can bind to the same ligands, CD152 binds to B71 and B72 with 20-100-fold higher affinity than CD28.

Product References
  1. Ex vivo coating of islet cell allografts with murine CTLA4/Fc promotes graft tolerance: W. Steurer, et al.; J. Immunol. 155, 1165 (1995)
  2. CTLA4Ig prevents lymphoproliferation and fatal multiorgan tissue destruction in CTLA-4-deficient mice : E.A. Tivol, et al.; J. Immunol. 158, 5091 (1997)
  3. Cutting edge: the related molecules CD28 and inducible costimulator deliver both unique and complementary signals required for optimal T cell activation: J.A. Gonzalo, et al.; J. Immunol. 166, 1 (2001)
  4. Recombinant Adenovirus Coexpressing Covalent Peptide/MHC Class II Complex and B7-1: In Vitro and In Vivo Activation of Myelin Basic Protein-Specific T Cells: J. Chen, et al.; J. Immunol. 167, 1297 (2001)
  5. Cutting Edge: Induced Indoleamine 2,3 Dioxygenase Expression in Dendritic Cell Subsets Suppresses T Cell Clonal Expansion: A.L. Mellor, et al.; J. Immunol. 171, 1652 (2003)
  6. Tim-3 inhibits T helper type 1-mediated auto- and alloimmune responses and promotes immunological tolerance: A. Sanchez-Fueyo, et al.; Nat. Immunol. 4, 1093 (2003)
  7. In Vitro and In Vivo Evaluation of Staphylococcal Superantigen Peptide Antagonists: G. Rajagopalan, et al.; Infect. Immun. v72, 6733 (2004)
  8. Blocking inducible co-stimulator in the absence of CD28 impairs Th1 and CD25+ regulatory T cells in murine colitis: Y.P. de Jong, et al.; Internat. Immunol. 16, 205 (2004)
  9. Specific subsets of murine dendritic cells acquire potent T cell regulatory functions following CTLA4-mediated induction of indoleamine 2,3 dioxygenase: A.L. Mellor, et al.; Internat. Immunol. 16, 1391 (2004)
  10. A mutant B7-1/Ig fusion protein that selectively binds to CTLA-4 ameliorates anti-tumor DNA vaccination and counters regulatory T cell activity: R. Chakrabarti, et al.; Vaccine 23, 4553 (2005)
  11. Programmed Death-1 Ligand 1 Interacts Specifically with the B7-1 Costimulatory Molecule to Inhibit T Cell Responses: M.J. Butte, et al.; Immunity 27, 111 (2007)
  12. Monovalent antibody scFv fragments selected to modulate T-cell activation by inhibition of CD86–CD28 interaction: R. Kolly, et al.; Prot. Engin. Des. Sel. 20, 91 (2007)
  13. Pivotal Advance: CTLA-4+ T cells exhibit normal antiviral functions during acute viral infection: H.-P. Raué & M.K. Slifka; J. Leuk. Biol. 81, 1165 (2007)
  14. Targeted Cleavage of Signaling Proteins by Caspase 3 Inhibits T Cell Receptor Signaling in Anergic T Cells: I. Puga, et al.; Immunity 29, 193 (2008)
  15. The Tumor Necrosis Factor-Family Receptor DR3 is Essential for Diverse T-Cell Mediated Inflammatory Diseases: F. Meylan, et al.; Immunity 29, 79 (2008)
  16. Improved immunological tolerance following combination therapy with CTLA-4/Ig and AAV-mediated PD-L1/2 muscle gene transfer: S. Adriouch, et al.; Front. Microbiol. 2, 199 (2011)
© 2017 Adipogen Life Sciences. Pictures: © 2012 Martin Oeggerli. All Rights Reserved.