IL-4 (mouse):Fc (mouse) (rec.) (non-lytic)

CHF 270.00
In stock
CHI-MF-12004-C01010 µgCHF 270.00
CHI-MF-12004-C05050 µgCHF 480.00
CHI-MF-12004-50105 x 10 µgCHF 945.00
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Product Details
Synonyms Interleukin-4; B Cell Stimulatory Factor 1; BSF-1
Product Type Protein
Properties
Source/Host CHO cells
Sequence

The extracellular domain of mouse IL-4 (aa 21-140) is fused to the N-terminus of the Fc region of a mutant mouse IgG2a.

Crossreactivity Mouse
Biological Activity

Shows the biological function of the IL-4 moiety and exerts a prolonged circulating half-life caused by the modified Fc domain.

Purity ≥98% (SDS-PAGE)
Endotoxin Content <0.06EU/μg protein (LAL test; Lonza).
Reconstitution Reconstitute at 100μg/ml in sterile PBS.
Formulation Lyophilized from 0.2μm-filtered solution in PBS.
Protein Negative Control

Fc (mouse) IgG2a Control (rec.) (non-lytic)

Other Product Data

Non-lytic: Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).

NCBI reference NP_067258.1: IL-4 (mouse)

Declaration Manufactured by Chimerigen.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

Interleukin-4 (IL-4) is a cytokine produced by type 2 helper T cells, the Th2 cells. These cells tends to make a specific set of lymphokines including IL-4, IL-5, IL-6, IL-10, IL-13, IL-3 and GM-CSF and fail to produce IL-2, IFN-γ, and lymphotoxin (TNF-β). In addition, mast cells can produce IL-4. IL-4 exerts numerous effects on various hematopoietic cell types. On B cells, IL-4 promotes immunoglobulin class switching to IgE and IgG1 isotypes and upregulates MHC class II and CD23 expression. IL-4 promotes survival, growth, and differentiation of both T and B lymphocytes, mast cells and endothelial cells. In addition, IL-4 inhibits the production of TNF, IL-1, and IL-6 by macrophages.

Product References
  1. Administration of noncytolytic IL-10/Fc in murine models of lipopolysaccharide-induced septic shock and allogeneic islet transplantation: X.X. Zheng, et al.; J. Immunol. 154, 5590 (1995)
  2. Prolonged islet allograft acceptance in the absence of interleukin 4 expression: P. Nickerson, et al.; Transpl. Immunol. 4, 81 (1996)
  3. Physiological mechanisms of regulating alloimmunity: cytokines, CTLA-4, CD25+ cells, and the alloreactive T cell clone size: M. Sho, et al.; J. Immunol. 169, 3744 (2002)
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