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RevMab
anti-Smac/Diablo (human), Rabbit Monoclonal (RM271)
459
CHF
CHF 459.00
In stock
REV-31-1152-00-R100100 µlCHF 459.00
Figure 1: Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Smac/Diablo Rabbit Monoclonal Antibody (Clone RM271) at a 1:1000 dilution.
| Product Details | |
|---|---|
| Synonyms | Direct IAP-binding Protein with low pI |
| Product Type | Recombinant Antibody |
| Properties | |
| Clone | RM271 |
| Isotype | Rabbit IgG |
| Source/Host | Rabbit |
| Immunogen/Antigen | A peptide corresponding to the C-terminus of human Smac (Diablo homolog, mitochondrial). |
| Application |
Immunohistochemistry (IHC): 1:500-1:1000 dilution |
| Crossreactivity | Human |
| Specificity |
This antibody reacts to human Smac (Diablo homolog, mitochondrial). |
| Purity | Protein A purified. |
| Purity Detail | Protein A affinity purified from an animal origin-free culture supernatant. |
| Concentration | N/A |
| Formulation | Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. |
| Isotype Negative Control | |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| Accession Number | Q9NR28 |
| Declaration | Manufactured by RevMab Biosciences. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Smac/Diablo is a 21 kDa mammalian mitochondrial protein that functions as a regulatory component during apoptosis. Upon mitochondrial stress, Smac/Diablo is released from mitochondria and competes with caspases for binding of IAPs (inhibitor of apoptosis proteins). The interaction of Smac/Diablo with IAPs relieves the inhibitory effect of the IAPs on caspases. This interaction involves mainly the amino-terminal residues of Smac/Diablo with the BIR3 region of XIAP, supplemented with several other hydrophobic interactions between the helical structures of Smac/Diablo and other areas of BIR3. Furthermore, Smac/DIABLO plays an important regulatory role in the sensitization of cancer cells to both immune-and drug-induced apoptosis. It promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway and acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP).