anti-Cyclin B1 (human), Rabbit Monoclonal (RM281)

Specifications / Handling

More Information
Product Details
Synonyms	CCNB1; G2/Mitotic-specific Cyclin-B1
Product Type	Recombinant Antibody
Properties
Clone	RM281
Isotype	Rabbit IgG
Source/Host	Rabbit
Immunogen/Antigen	A peptide corresponding to Cyclin B1.
Application	Immunohistochemistry (IHC): 1:1000-1:2000 dilution Western Blot (WB): 1:1000-1:2000 dilution
Crossreactivity	Human
Specificity	This antibody reacts to human Cyclin B1.
Purity	Protein A purified.
Purity Detail	Protein A affinity purified from an animal origin-free culture supernatant.
Concentration	N/A
Formulation	Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide.
Isotype Negative Control	Rabbit IgG
Other Product Data	Click here for Original Manufacturer Product Datasheet Our product description may differ slightly from the original manufacturers product datasheet.
Accession Number	P14635
Declaration	Manufactured by RevMab Biosciences.
Shipping and Handling
Shipping	BLUE ICE
Long Term Storage	-20°C
Handling Advice	Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Documents
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Cyclins bind to and regulate the activity of the Cyclin dependent protein kinases (CDKs). Cyclin B1 or CCNB1 is a regulatory protein involved in mitosis. It is essential for the control of the cell cycle at the G2/M transition. Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. Cyclin B1 is not ubiquitinated during G2/M phase, resulting in its steady accumulation during G2 phase, followed by abrupt APC-dependent destruction at the end of mitosis. Destruction of Cyclin B1 is required for cell cycle progression. Cyclin B1 is overexpressed in various cancers, including breast, prostate and non-small cell lung cancer.

Call	+41 61 926 6040
Fax	+41 61 926 6049