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RevMab
anti-SOX2 (human), Rabbit Monoclonal (RM427)
459
CHF
CHF 459.00
In stock
REV-31-1314-00-R100100 µlCHF 459.00
Figure 1: Immunohistochemical staining of formalin fixed and paraffin embedded human lung squamous Cell Carcinoma tissue section using anti-SOX2 rabbit monoclonal antibody (Clone RM427) at a 1:100 dilution.
| Product Details | |
|---|---|
| Synonyms | SRY-related HMG-box Gene 2; SRY (Sex Determining Region Y)-Box 2; MCOPS3; ANOP3 |
| Product Type | Recombinant Antibody |
| Properties | |
| Clone | RM427 |
| Isotype | Rabbit IgG |
| Source/Host | Rabbit |
| Immunogen/Antigen | A peptide corresponding to the C-terminus of human SOX2. |
| Application |
Immunohistochemistry (IHC): 1:100-1:250 dilution Western Blot (WB): 1:100-1:2000 dilution |
| Crossreactivity | Human |
| Specificity |
RM427 reacts to human SOX2. |
| Purity | Protein A purified. |
| Purity Detail | Protein A affinity purified from an animal origin-free culture supernatant. |
| Concentration | N/A |
| Formulation | Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. |
| Isotype Negative Control | |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| Accession Number | P48431 |
| Declaration | Manufactured by RevMab Biosciences. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
SOX2 is the most studied member of SRY-related box transcription factor family. It binds to target genes through its highly conserved HMG box domain. Inactivation of the SOX2 gene causes lethality during embryonic development. SOX2 knockdown in embryonic stem cells results in their differentiation. Co-expression of SOX with OCT4, MYC, and KLF4 is sufficient to reprogram somatic cells to induced pluripotent stem cells (iPSCs), which exert similar characteristics as natural pluripotent stem cells. These findings indicate that SOX2 is crucial for the self-renewal and pluripotency of embryonic stem cells. In addition, over-expression of SOX2 has been found in various types of malignant cancer. Knockdown of SOX2 results in cell cycle arrest by downregulating cyclin D1 and inhibition of tumor cell proliferation, suggesting that SOX2 is involved in activating genes associated with tumor progression.