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RevMab
anti-DDIT3 (human), Rabbit Monoclonal (RM485)
459
CHF
CHF 459.00
In stock
REV-31-1377-00-R100100 µlCHF 459.00
Figure 1: Western Blot of SW620 cell lysate using anti-DDIT3 rabbit monoclonal antibody (Clone RM485) at a 1:1000 dilution.
| Product Details | |
|---|---|
| Synonyms | DNA Damage-inducible Transcript 3 Protein; C/EBP-homologous Protein 10; CHOP-10; GADD153 |
| Product Type | Recombinant Antibody |
| Properties | |
| Clone | RM485 |
| Isotype | Rabbit IgG |
| Source/Host | Rabbit |
| Immunogen/Antigen | A peptide corresponding to the N-terminus of human DDIT3 (DNA damage-inducible transcript 3 protein). |
| Application |
Western Blot (WB): 1:1000-1:2000 dilution |
| Crossreactivity | Human |
| Specificity |
This antibody reacts to human DDIT3 (DNA damage-inducible transcript 3 protein). |
| Purity | Protein A purified. |
| Purity Detail | Protein A affinity purified from an animal origin-free culture supernatant. |
| Concentration | N/A |
| Formulation | Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. |
| Isotype Negative Control | |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| Accession Number | P35638 |
| Declaration | Manufactured by RevMab Biosciences. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
DDIT3 [GADD153] is a small nuclear protein that is capable of dimerizing with transcription factors C/EBP alpha and beta. Once dimerized, this complex inhibits the normal binding and function of C/EBP to classical binding sites. Inversely, the C/EBP DDIT3 dimer gains binding activity to other non classical C/EBP stress related targets. Under normal cellular conditions this protein is not expressed in detectable levels, but is highly unregulated during times of cellular/ER stress. Examples of DDIT3 inducing stress include: treatment with tunicamycin, nutrient starvation and reducing agents that interfere with the calcium flux across the ER membrane. DDIT3 is a multifunctional transcription factor in endoplasmic reticulum (ER) stress response that plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress.