Ubiquitin Activating Enzyme E1 [UBE1] (human) (rec.) (untagged)
|Synonyms||Ubiquitin E1 Enzyme; Ubiquitin-like Modifier-activating Enzyme 1; A1S9T; AMCX1; GXP1; POC20; SMAX2|
|Sequence||Human UBA1 (aa1-2-1058) (Accession Nr. P22314) is untagged.|
|Application||Typical working concentration range is 10-100nM.|
|Formulation||Liquid. In 50mM HEPES pH 7.5, 150mM sodium chloride, 10% glycerol, 2mM TCEP.|
|Other Product Data||
Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer
Our product description may differ slightly from the original manufacturers product datasheet.
|Declaration||Manufactured by South Bay Bio.|
|Shipping and Handling|
|Short Term Storage||-80°C|
|Long Term Storage||-80°C|
|Handling Advice||Aliquot to avoid freeze/thaw cycles.|
|Use/Stability||Stable for at least 1 year after receipt when stored at -80°C.|
|Product Specification Sheet|
UBA1, the canonical ubiquitin E1 activating enzyme is a 118kDa protein, which forms a homodimer in its active state and catalyzes the first step of the ubiquitin conjugation cascade. It activates ubiquitin in an ATP-dependent mechanism where ATP is hydrolyzed to AMP and PPi, a ubiquitin C-terminal adenylate intermediate is formed, then transferred to the E1’s active site cysteine through a thioester bond. This thioester is then transferable to an E2 conjugating enzyme’s active site cysteine.