SouthBayBio
Ubiquitin Activating Enzyme E1 [UBE1] (human) (rec.) (untagged)
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CHF 356.00
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SBB-CE0011-C05050 µgCHF 356.00
| Product Details | |
|---|---|
| Synonyms | Ubiquitin E1 Enzyme; Ubiquitin-like Modifier-activating Enzyme 1; A1S9T; AMCX1; GXP1; POC20; SMAX2 |
| Product Type | Protein |
| Properties | |
| Source/Host | Sf21 cells |
| Sequence | Human UBA1 (aa1-2-1058) (Accession Nr. P22314) is untagged. |
| Crossreactivity | Human |
| Application | Typical working concentration range is 10-100nM. |
| MW | ~118kDa |
| Purity | ≥95% (SDS-PAGE) |
| Concentration | Lot dependent. |
| Accession Number | P22314 |
| Formulation | Liquid. In 50mM HEPES pH 7.5, 150mM sodium chloride, 10% glycerol, 2mM TCEP. |
| Other Product Data |
Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer |
| Declaration | Manufactured by South Bay Bio. |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -80°C |
| Long Term Storage | -80°C |
| Handling Advice | Aliquot to avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -80°C. |
| Documents | |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
UBA1, the canonical ubiquitin E1 activating enzyme is a 118kDa protein, which forms a homodimer in its active state and catalyzes the first step of the ubiquitin conjugation cascade. It activates ubiquitin in an ATP-dependent mechanism where ATP is hydrolyzed to AMP and PPi, a ubiquitin C-terminal adenylate intermediate is formed, then transferred to the E1’s active site cysteine through a thioester bond. This thioester is then transferable to an E2 conjugating enzyme’s active site cysteine.