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SouthBayBio
UbcH7 [UBE2L3] (human) (rec.) (untagged)
345
CHF
CHF 345.00
In stock
SBB-CE0020-C100100 µgCHF 345.00
Figure 1: Thioester Activity Assay: UBE2L3 (UbcH7) forms a thioester with Ub in an ATP-dependent manner and the bond can be reduced with addition of access DTT. Confirms the activity of UBE2L3.
| Product Details | |
|---|---|
| Synonyms | Ubiquitin-conjugating Enzyme E2 L3; E2-F1; L-UBC; UBCE7; UbcM4; UBE2L3; Ubiquitin-protein Ligase L3 |
| Product Type | Protein |
| Properties | |
| Source/Host | E. coli |
| Sequence | Human UBE2L3 (aa 1-154) (Accession Nr. P68036) is untagged. |
| Crossreactivity | Human |
| Application | Typical working concentration range is 1-5µM. |
| MW | ~18kDa |
| Purity | ≥95% (SDS-PAGE) |
| Concentration | Lot dependent. |
| Accession Number | P68036 |
| Formulation | Liquid. In 50mM HEPES pH 7.5, 150mM sodium chloride, 10% glycerol, 2mM TCEP. |
| Other Product Data |
Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer Our product description may differ slightly from the original manufacturers product datasheet. |
| Declaration | Manufactured by South Bay Bio. |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -80°C |
| Long Term Storage | -80°C |
| Handling Advice | Aliquot to avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -80°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
UBE2L3 (UbcH7) is an E2 ubiquitin conjugating enzyme and accepts ubiquitin from an E1 activating enzyme via an active site cysteine. The mechanism of ubiquitin transfer involves the breaking of a E1-Ub thioester linkage, followed by a reformation of a UBE2L3-Ub thioester. UBE2L3 transfers active ubiquitin molecules via a final transthiolation reaction to a cysteine residue of HECT class or RBR class E3 ligases. UBE2L3 plays a critical role in the activation of NF-κb signaling by working in conjugation with the RBR E3 ligase, LUBAC (linear ubiquitin assembly complex), to ubiquitinate NEMO of the IκB complex, which than facilitates the downstream immune response.