UBE2L3 [UbcH7] (human) (rec.) (untagged)

CHF 258.00
In stock
SBB-CE0020-C100100 µgCHF 258.00
 
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Product Details
Synonyms Ubiquitin-conjugating Enzyme E2 L3; E2-F1; L-UBC; UBCE7; UbcM4; UBE2L3; Ubiquitin-protein Ligase L3
Product Type Protein
Properties
Source/Host E. coli
Sequence Human UBE2L3 (aa 1-154) (Accession Nr. P68036) is untagged.
Crossreactivity Human
Application Typical working concentration range is 1-5µM.
MW ~18kDa
Purity ≥95% (SDS-PAGE)
Concentration Lot dependent.
Accession Number P68036
Formulation Liquid. In 50mM HEPES pH 7.5, 150mM sodium chloride, 10% glycerol, 2mM TCEP.
Other Product Data Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer
Our product description may differ slightly from the original manufacturers product datasheet.
Declaration Manufactured by South Bay Bio.
Shipping and Handling
Shipping DRY ICE
Short Term Storage -80°C
Long Term Storage -80°C
Handling Advice Aliquot to avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -80°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF
UBE2L3 (UbcH7) is an E2 ubiquitin conjugating enzyme and accepts ubiquitin from an E1 activating enzyme via an active site cysteine. The mechanism of ubiquitin transfer involves the breaking of a E1-Ub thioester linkage, followed by a reformation of a UBE2L3-Ub thioester. UBE2L3 transfers active ubiquitin molecules via a final transthiolation reaction to a cysteine residue of HECT class or RBR class E3 ligases. UBE2L3 plays a critical role in the activation of NF-κb signaling by working in conjugation with the RBR E3 ligase, LUBAC (linear ubiquitin assembly complex), to ubiquitinate NEMO of the IκB complex, which than facilitates the downstream immune response.
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