UbcH5a [UBE2D1] (human) (rec.) (untagged)
SBB-CE0021-C100100 µgCHF 258.00
Thioester Activity Assay: UBE2D1 (UbcH5a) forms a thioester with Ub in an ATP-dependent manner and the bond can be reduced with addition of access DTT. Confirms the activity of UBE2D1.
|Synonyms||Ubiquitin-conjugating Enzyme H5a; PUBC1; UBC5A; Ubiquitin-protein Ligase D1; p53-regulated Ubiquitin-conjugating Enzyme 1|
|Sequence||Human UBE2D1 (aa1-147) (Accession Nr. P51668) is untagged.|
|Application||Typical working concentration range is 1-5µM.|
|Formulation||Liquid. In 50mM HEPES pH 7.5, 150mM sodium chloride, 10% glycerol (v/v), 2mM TCEP.|
|Other Product Data||
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|Declaration||Manufactured by South Bay Bio.|
|Shipping and Handling|
|Short Term Storage||-80°C|
|Long Term Storage||-80°C|
|Handling Advice||Aliquot to avoid freeze/thaw cycles.|
|Use/Stability||Stable for at least 1 year after receipt when stored at -80°C.|
|Product Specification Sheet|
UBE2D1 (UbcH5a) is an E2 ubiquitin conjugating enzyme. An E1 activating enzyme is required to attach ubiquitin to UBE2D1 via an active site cysteine. The mechanism of ubiquitin transfer involves the breaking of a E1-Ub thioester linkage, followed by a reformation of a UBE2D1-Ub thioester. UBE2D1 is capable of associating with numerous known E3 ligases which target abnormal proteins for proteasomal degradation through polyubiquitination. UBE2D1 is also known to interact with Parkin and to be involved in PINK1 mediated mitophagy.