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SouthBayBio
UbcH5a [UBE2D1] (human) (rec.) (untagged)
345
CHF
CHF 345.00
In stock
SBB-CE0021-C100100 µgCHF 345.00
Figure 1: Thioester Activity Assay: UBE2D1 (UbcH5a) forms a thioester with Ub in an ATP-dependent manner and the bond can be reduced with addition of access DTT. Confirms the activity of UBE2D1.
| Product Details | |
|---|---|
| Synonyms | Ubiquitin-conjugating Enzyme H5a; PUBC1; UBC5A; Ubiquitin-protein Ligase D1; p53-regulated Ubiquitin-conjugating Enzyme 1 |
| Product Type | Protein |
| Properties | |
| Source/Host | E. coli |
| Sequence | Human UBE2D1 (aa1-147) (Accession Nr. P51668) is untagged. |
| Crossreactivity | Human |
| Application | Typical working concentration range is 1-5µM. |
| MW | ~17kDa |
| Purity | ≥95% (SDS-PAGE) |
| Concentration | Lot dependent. |
| Accession Number | P51668 |
| Formulation | Liquid. In 50mM HEPES pH 7.5, 150mM sodium chloride, 10% glycerol (v/v), 2mM TCEP. |
| Other Product Data |
Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer Our product description may differ slightly from the original manufacturers product datasheet. |
| Declaration | Manufactured by South Bay Bio. |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -80°C |
| Long Term Storage | -80°C |
| Handling Advice | Aliquot to avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -80°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
UBE2D1 (UbcH5a) is an E2 ubiquitin conjugating enzyme. An E1 activating enzyme is required to attach ubiquitin to UBE2D1 via an active site cysteine. The mechanism of ubiquitin transfer involves the breaking of a E1-Ub thioester linkage, followed by a reformation of a UBE2D1-Ub thioester. UBE2D1 is capable of associating with numerous known E3 ligases which target abnormal proteins for proteasomal degradation through polyubiquitination. UBE2D1 is also known to interact with Parkin and to be involved in PINK1 mediated mitophagy.