UbcH1 [UBE2K, E2-25K] (human) (rec.) (His)
SBB-CE0022-C100100 µgCHF 258.00
Thioester Activity Assay: UBE2K forms a thioester with Ub in an ATP-dependent manner and the bond can be reduced with addition of excess DTT. The thioester assay also shows di-ubiquitin formation with addition of ATP. This confirms the activity of UBE2K.
|Synonyms||Ubiquitin-conjugating Enzyme E2; E2-25K; Hip2; HYPG; LIG; UBE2K; UBE2K-25K; Huntingtin-interacting Protein 2|
|Sequence||Human UBE2K (aa 1-200) (Accession Nr. P61086) is fused at the N-terminus to a His-tag.|
|Application||Typical working concentration range is 1-5µM.|
|Formulation||Liquid. In 50mM HEPES pH 7.5, 150mM sodium chloride, 10% glycerol (v/v), 2mM TCEP.|
|Other Product Data||
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|Declaration||Manufactured by South Bay Bio.|
|Shipping and Handling|
|Short Term Storage||-80°C|
|Long Term Storage||-80°C|
|Handling Advice||Aliquot to avoid freeze/thaw cycles.|
|Use/Stability||Stable for at least 1 year after receipt when stored at -80°C.|
|Product Specification Sheet|
UBE2K (UbcH1) is an E2 ubiquitin conjugating enzyme. An E1 activating enzyme is required to attach ubiquitin to UBE2K via an active site cysteine. The mechanism of ubiquitin transfer involves the breaking of a E1-Ub thioester linkage, followed by a reformation of a UBE2K-Ub thioester. UBE2K is capable of synthesizing K48-linked ubiquitin chains and can do so without an E3 ubiquitin ligating enzyme present. UBE2K catalyzes free K48-linked polyubiquitin chains and is known to interact with huntingtin and TRIM6. Although an E3 ligase is not required for chain formation, free UBE2K synthesized K48 polyubiquitin chains have been shown to interact with the E3 TRIM6, leading to activation of IKKε kinase activity and subsequent antiviral activity.