UbcH1 [UBE2K, E2-25K] (human) (rec.) (His)

CHF 345.00
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SBB-CE0022-C100100 µgCHF 345.00
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Product Details
Synonyms Ubiquitin-conjugating Enzyme E2; E2-25K; Hip2; HYPG; LIG; UBE2K; UBE2K-25K; Huntingtin-interacting Protein 2
Product Type Protein
Source/Host E. coli
Sequence Human UBE2K (aa 1-200) (Accession Nr. P61086) is fused at the N-terminus to a His-tag.
Crossreactivity Human
Application Typical working concentration range is 1-5µM.
MW ~22kDa (SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Concentration Lot dependent.
Accession Number P61086
Formulation Liquid. In 50mM HEPES pH 7.5, 150mM sodium chloride, 10% glycerol (v/v), 2mM TCEP.
Other Product Data Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer
Our product description may differ slightly from the original manufacturers product datasheet.
Declaration Manufactured by South Bay Bio.
Shipping and Handling
Shipping DRY ICE
Short Term Storage -80°C
Long Term Storage -80°C
Handling Advice Aliquot to avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -80°C.
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

UBE2K (UbcH1) is an E2 ubiquitin conjugating enzyme. An E1 activating enzyme is required to attach ubiquitin to UBE2K via an active site cysteine. The mechanism of ubiquitin transfer involves the breaking of a E1-Ub thioester linkage, followed by a reformation of a UBE2K-Ub thioester. UBE2K is capable of synthesizing K48-linked ubiquitin chains and can do so without an E3 ubiquitin ligating enzyme present. UBE2K catalyzes free K48-linked polyubiquitin chains and is known to interact with huntingtin and TRIM6. Although an E3 ligase is not required for chain formation, free UBE2K synthesized K48 polyubiquitin chains have been shown to interact with the E3 TRIM6, leading to activation of IKKε kinase activity and subsequent antiviral activity.

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