UcH-L3 (human) (rec.) (untagged)
SBB-DE0023-C05050 µgCHF 155.00
Michaelis–Menten Kinetics: Ubiquitin Rhodamine 110 serially diluted from 1.6 to 0.05µM was digested with 30pM UcHL3 over time. The assay was carried out in a reaction buffer of 50mM HEPES pH 7.5, 100mM NaCl, 1mM TCEP, 0.1mg/ml BSA, at 25°C. Initial velocities at each substrate concentration were plotted and fit to the Michaelis–Menten equation. Kinetic parameters were calculated at: Km= 0.69μM, Vmax = 1.29 nMs-1, kcat= 43s-1, kcat/Km= 6.16 x 107 M-1s-1.
|Synonyms||Ubiquitin C-terminal Hydrolase L3; Ubiquitin Thioesterase L3|
|Sequence||Human UcH-L3 (aa 1-230) (Accession Nr. P15374) is untagged.|
|Application||Typical working concentration range is 0.5-5nM.|
|Formulation||Liquid. In 50mM HEPES pH 7.5, 100mM sodium chloride, 1mM TCEP.|
|Other Product Data||
Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer
Our product description may differ slightly from the original manufacturers product datasheet.
|Declaration||Manufactured by South Bay Bio.|
|Shipping and Handling|
|Short Term Storage||-80°C|
|Long Term Storage||-80°C|
|Handling Advice||Aliquot to avoid freeze/thaw cycles.|
|Use/Stability||Stable for at least 1 year after receipt when stored at -80°C.|
|Product Specification Sheet|
UCHL3 (Ubiquitin C-terminal Hydrolase L3) is a deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. It is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. It plays a role in regulating apical membrane recycling and indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. It is required for stress-response in retinal, skeletal muscle and germ cell maintenance. UCHL3 is also known to hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders. It digests precursors and ubiquitinated proteins to generate monomeric ubiquitin.