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SouthBayBio
UcH-L3 (human) (rec.) (untagged)
278
CHF
CHF 278.00
In stock
SBB-DE0023-C05050 µgCHF 278.00
Figure 1: Michaelis–Menten Kinetics: Ubiquitin Rhodamine 110 serially diluted from 1.6 to 0.05µM was digested with 30pM UcHL3 over time. The assay was carried out in a reaction buffer of 50mM HEPES pH 7.5, 100mM NaCl, 1mM TCEP, 0.1mg/ml BSA, at 25°C. Initial velocities at each substrate concentration were plotted and fit to the Michaelis–Menten equation. Kinetic parameters were calculated at: Km= 0.69μM, Vmax = 1.29 nMs-1, kcat= 43s-1, kcat/Km= 6.16 x 107 M-1s-1.
| Product Details | |
|---|---|
| Synonyms | Ubiquitin C-terminal Hydrolase L3; Ubiquitin Thioesterase L3 |
| Product Type | Protein |
| Properties | |
| Source/Host | E. coli |
| Sequence | Human UcH-L3 (aa 1-230) (Accession Nr. P15374) is untagged. |
| Crossreactivity | Human |
| Application | Typical working concentration range is 0.5-5nM. |
| MW | ~26kDa |
| Purity | ≥95% (SDS-PAGE) |
| Concentration | Lot dependent. |
| Accession Number | P15374 |
| Formulation | Liquid. In 50mM HEPES pH 7.5, 100mM sodium chloride, 1mM TCEP. |
| Other Product Data |
Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer Our product description may differ slightly from the original manufacturers product datasheet. |
| Declaration | Manufactured by South Bay Bio. |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -80°C |
| Long Term Storage | -80°C |
| Handling Advice | Aliquot to avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -80°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
UCHL3 (Ubiquitin C-terminal Hydrolase L3) is a deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. It is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. It plays a role in regulating apical membrane recycling and indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. It is required for stress-response in retinal, skeletal muscle and germ cell maintenance. UCHL3 is also known to hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders. It digests precursors and ubiquitinated proteins to generate monomeric ubiquitin.