SouthBayBio

Ubiquitin (human) (rec.) (Rhodamine 110)

As low as CHF 363.00

In stock

Only %1 left

SBB-PS0001-C05050 µgCHF 363.00

Figure 1: Signal to Background: The signal to background ratio was determined by 100% hydrolysis of either 50nM or 500nM Ubiquitin-Rhodamine 110 to liberate the quenched conjugate. Assay Buffer: 50mM HEPES pH7.5, 100mM NaCl, 1mM TCEP, 0.1mg/ml BSA.

Figure 2: Robustness of Rhodamine 110 vs. 7-amino-4- methylcoumarin (AMC) substrates: Fluorescent substrates (500nM Ub-Rh110/Ub-AMC) were incubated +/- 5pM UcHL3 in a 384 well plate (n=16), and progress curves were normalized to the maximum fluorescence signal to produce “% reaction progress”. The Z’ value was calculated at each time-point.

Specifications / Handling

More Information
Product Details
Synonyms	UBB; Ubiquitin B
Product Type	Protein
Properties
Source/Host	E. coli
Sequence	Human ubiquitin (aa1-76) (Accession Nr. P0CG47) conjugated at the C-terminus to a quenched Rhodamine 110 dye.
Crossreactivity	Human
Application	Protein-based substrate. Typical working concentration range is 50-500nM. Hydrolysis of the conjugate results in fluorescence observable by excitation at 485nm and emission at 535nm.
Label/Conjugates	Rhodamine
MW	~9kDa
Purity	≥99% (LCMS)
Concentration	Lot dependent.
Accession Number	P0CG47
Formulation	Liquid. In 50mM MES pH 6.0.
Other Product Data	Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer Our product description may differ slightly from the original manufacturers product datasheet.
Declaration	Manufactured by South Bay Bio.
Shipping and Handling
Shipping	DRY ICE
Short Term Storage	-80°C
Long Term Storage	-80°C
Handling Advice	Aliquot to avoid freeze/thaw cycles. Protect from light.
Use/Stability	Stable for at least 1 year after receipt when stored at -80°C.
Documents
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Ubiquitin is a 76 amino acid post-translational modifier expressed throughout all tissues in eukaryotic organisms. The many roles of ubiquitin modification include proteasomal degradation, signal transduction, inflammatory response and DNA damage repair. Ubiquitin modification occurs through a pyramidal cascade of an E1 activating enzyme, E2 conjugating enzymes and an E3 ubiquitin ligase. This enzymatic cascade results in modification of a ε-amine of a lysine residue on a substrate protein. Substrates may either be mono- or poly-ubiquitinated by M1, K6, 11, 27, 29, 33, 48 or 63 linkages. Removal of ubiquitin from a substrate protein occurs via deconjugating enzymes, of which there are nearly 100 known enzymes with various specificities.

Call	+41 61 926 6040
Fax	+41 61 926 6049