Ubiquitin (human) (rec.) (Rhodamine 110)
|Synonyms||UBB; Ubiquitin B|
|Sequence||Human ubiquitin (aa1-76) (Accession Nr. P0CG47) conjugated at the C-terminus to a quenched Rhodamine 110 dye.|
|Application||Protein-based substrate. Typical working concentration range is 50-500nM. Hydrolysis of the conjugate results in fluorescence observable by excitation at 485nm and emission at 535nm.|
|Formulation||Liquid. In 50mM MES pH 6.0.|
|Other Product Data||
Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer
Our product description may differ slightly from the original manufacturers product datasheet.
|Declaration||Manufactured by South Bay Bio.|
|Shipping and Handling|
|Short Term Storage||-80°C|
|Long Term Storage||-80°C|
Aliquot to avoid freeze/thaw cycles.
Protect from light.
|Use/Stability||Stable for at least 1 year after receipt when stored at -80°C.|
|Product Specification Sheet|
Ubiquitin is a 76 amino acid post-translational modifier expressed throughout all tissues in eukaryotic organisms. The many roles of ubiquitin modification include proteasomal degradation, signal transduction, inflammatory response and DNA damage repair. Ubiquitin modification occurs through a pyramidal cascade of an E1 activating enzyme, E2 conjugating enzymes and an E3 ubiquitin ligase. This enzymatic cascade results in modification of a ε-amine of a lysine residue on a substrate protein. Substrates may either be mono- or poly-ubiquitinated by M1, K6, 11, 27, 29, 33, 48 or 63 linkages. Removal of ubiquitin from a substrate protein occurs via deconjugating enzymes, of which there are nearly 100 known enzymes with various specificities.