p53 (polyubiquitinated) (human) (rec.) (His)
SBB-US0012-C02020 µgCHF 416.00
Ubiquitinated-p53 Western Blot: From left to right: Control His6-p53 (50ng), ubiquitinated-p53 (50ng and 100ng) and ubiquitinated-p53 digested with USP7 (50ng).
|Synonyms||Cellular Tumor Antigen p53; Tumor Suppressor p53; TP53|
|Sequence||Human p53 (aa 2-393) (Accession Nr. P04637) fused to a N-terminal His-tag, ubiquitinated by MDM2, and subsequently purified from conjugation-reaction proteins/enriched post-conjugation.|
|Application||Typical working concentrations range is 0.5-2µM.|
|MW||>40kDa. Western blot analysis shows multiple states of poly-ubiquitination, and deconjugation with USP7 shows purified ubiquitinated p53 can function as a native substrate.|
|Formulation||Liquid. In 50mM HEPES pH 7.5, 500 mM sodium chloride, 10% glycerol, 2mM TCEP.|
|Other Product Data||
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|Declaration||Manufactured by South Bay Bio.|
|Shipping and Handling|
|Short Term Storage||-80°C|
|Long Term Storage||-80°C|
|Handling Advice||Aliquot to avoid freeze/thaw cycles.|
|Use/Stability||Stable for at least 1 year after receipt when stored at -80°C.|
|Product Specification Sheet|
p53 acts as a tumor suppressor in many tumor types, induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. E3 ligase MDM2 mediated ubiquitin conjugation of p53 and subsequent p53 down-regulation via proteasomal degradation interrupts this crucial role, contributing to tumorogenesis (and cancer).