ImmPRESS™ anti-Rabbit IgG Kit, AP Alkaline Phosphatase

Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS™ Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.   Learn more about the ImmPRESS™ Polymer Detection Systems in double labeling.   The ImmPRESS™ polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step, biotin-free detection system. This unique micropolymer of highly active enzyme (peroxidase or alkaline phosphatase) is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background.     The ImmPRESS™-AP Alkaline Phosphatase Polymer Reagent micropolymer technology limits steric interference and provides enhanced accessibility to the target avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones.  The result is crisp, strong staining of antibody targets, especiallly nuclear and membrane antigens, and  greater sensitivity than conventional alkaline phosphatase antibody conjugates and other alkaline phosphatase polymer systems for immunohistochemistry and other applications.  Key advantages of the ImmPRESS™ Polymer System: High sensitivity, Low background, Non-biotin Micropolymer technology, Enhanced accessibility to nuclear and membrane antigens, One-step detection with shorter assay time, Ready-to-use in convenient dropper bottle with blocking solution included Simplified multiple labeling Staining Procedure. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS™ Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice. Consider Species Cross-Reactivity When choosing the optimal detection system for your application, it is important to consider not only the species of the primary antibody but also the species of the tissue. If the species of the primary antibody and the species of the tissue are closely related (e.g. rat and mouse), the secondary antibody may bind to endogenous IgG in the tissue section leading to background.

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