VECTASTAIN Elite ABC Universal PLUS Kit, Peroxidase (Horse Anti-Mouse/Rabbit IgG)

Specifications / Handling

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Product Details
Product Type	Kit
Properties
Application Set	Compound Screening
Detection Type	Fluorescent
Kit Contains	50 ml ready-to-use stabilized Elite® ABC reagent 50 ml ready-to-use universal, biotinylated anti-mouse/rabbit IgG 50 ml 2.5% normal horse serum 50 ml BLOXALL Endogenous Enzyme Blocking Solution ImmPACT DAB EqV Substrate (25 ml Reagent 1 + 25 ml Reagent 2) Sufficient reagents to stain approximately 250-500 sections.
Other Product Data	VECTASTAIN Elite ABC System Advanced avidin-biotin technology: The Elite ABC complex is smaller, very uniform, and highly active, allowing more accessibility for binding to a biotinylated target Highest sensitivity, low background: The VECTASTAIN Elite ABC system is the most sensitive avidin-biotin complex-based peroxidase system. The Elite ABC series is approximately 5 times more sensitive than the original VECTASTAIN ABC Kit with the same low background. Cost effective: Higher sensitivity leads to lower cost per slide. Available without ( Standard kit) or with biotinylated species specific or universal secondary antibodies. Available in Ready-To-Use (R.T.U.) formats: Prediluted, stabilized working solutions of Elite ABC Kit reagents provide the same high sensitivity and low background as the traditional VECTASTAIN Elite ABC Staining Kit reagents. Note: Our Universal Biotinylated Anti-Mouse/Rabbit IgG secondary antibody included in this kit is a cocktail of biotinylated anti-mouse IgG and anti-rabbit IgG, designed for use with both rabbit and mouse primary antibodies. This antibody should not be used to stain tissues from rodents or rabbits due to its reactivity with IgG present in the tissues of these species. Click here for Original Manufacturer Product Datasheet and Complete Product Information
Declaration	Manufactured by Vector Laboratories
Shipping and Handling
Shipping	AMBIENT
Short Term Storage	+20°C
Long Term Storage	+4°C
Handling Advice	Do not freeze.
Documents
Manual	No
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Protein chromogenic detection reagent used for Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs.

With more than 50,000 citations to its credit, the VECTASTAIN ABC kit remains widely popular. Based on the versatile avidin-biotin complex interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. Our ABC kits are economical and continue to be a staple product in any immunohistochemistry (IHC) and immunocytochemistry (ICC) laboratory.

Peroxidase substrates produce sharp, dense precipitates with crisp localization. These characteristics, in conjunction with the high sensitivity and low background of the VECTASTAIN ABC systems, make the peroxidase enzyme a preferred choice in many applications. (eg. In neural tissue, the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes.)

The Elite ABC complex is smaller, very uniform, and highly active, allowing more accessibility for binding to a biotinylated target

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