anti-Adiponectin (human), mAb (HADI 773)
AG-20A-0001-C05050 µgCHF 270.00
AG-20A-0001-C100100 µgCHF 350.00
Immunohistochemical staining of adiponectin using anti-Adiponectin (human), mAb (HADI 773) (Prod. No. AG-20A-0001) in formalin-fixed and paraffin-embedded (FFPE) human kidney tissue (10µg/ml).
Western blot analysis of human plasma adiponectin using anti-Adiponectin (human), mAb (HADI 773) (Prod. No. AG-20A-0001) at 0.2µg/ml.
|Synonyms||ACRP30; AdipoQ; apM1; GBP28; Adipocyte Complement Related Protein of 30kDa|
|Product Type||Monoclonal Antibody|
|Immunogen/Antigen||Recombinant human adiponectin.|
|Application||ELISA: (direct and indirect: 1:5'000-1:10'000).
Immunohistochemistry: (paraffin sections: 1:500 in TBS/Triton for 30 min. at room temperature).
Western blot: (1:5'000-1:10'000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
|Specificity||Recognizes human adiponectin. Detects a band of ~30kDa by Western blot. Cross-reactivity with other species not tested.|
|Purity Detail||Protein G-affinity purified.|
|Formulation||Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
|Handling Advice||After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
|Use/Stability||Stable for at least 1 year after receipt when stored at -20°C.|
|Product Specification Sheet|
ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels.
- Plasma resistin concentrations measured by enzyme-linked immunosorbent assay using a newly developed monoclonal antibody are elevated in individuals with type 2 diabetes mellitus: B.S. Youn, et al.; J. Clin. Endocrinol. Metab. 89, 150 (2004)
- Genetic association study of adiponectin polymorphisms with risk of Type 2 diabetes mellitus in Korean population: Y.Y. Lee, et al.; Diabet. Med. 22, 569 (2005)
- Adiponectin expression in human fetal tissues during mid- and late gestation: S. Corbetta, et al,; J. Clin. Endocrinol. Metab. 90, 2397(2005)
- Renin receptor expression in human adipose tissue: V. Achard, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 292, R274 (2007)
- Adiponectin immunohistochemical expression in colorectal cancer and its correlation with histological grade and tumour microvessel density: V. Barresi, et al.; Pathology 41, 533 (2009)
- The expression of adiponectin receptors Adipo-R1 and Adipo-R2 is associated with an intestinal histotype and longer survival in gastric carcinoma: V. Barresi, et al.; J. Clin. Pathol. 62, 705 (2009)