AdipoGen Life Sciences

anti-Adiponectin (human), mAb (HADI 773)

CHF 270.00

In stock

AG-20A-0001-C05050 µgCHF 270.00

AG-20A-0001-C100100 µgCHF 350.00

Figure 1: Immunohistochemical staining of adiponectin using anti-Adiponectin (human), mAb (HADI 773) (Prod. No. AG-20A-0001) in formalin-fixed and paraffin-embedded (FFPE) human kidney tissue (10µg/ml).

Figure 2: Western blot analysis of human plasma adiponectin using anti-Adiponectin (human), mAb (HADI 773) (Prod. No. AG-20A-0001) at 0.2µg/ml.

Specifications / Handling

More Information
Product Details
Synonyms	ACRP30; AdipoQ; apM1; GBP28; Adipocyte Complement Related Protein of 30kDa
Product Type	Monoclonal Antibody
Properties
Clone	HADI 773
Isotype	Mouse IgG1κ
Immunogen/Antigen	Recombinant human adiponectin.
Application	ELISA: (direct and indirect: 1:5'000-1:10'000). Immunohistochemistry: (paraffin sections: 1:500 in TBS/Triton for 30 min. at room temperature). Western blot: (1:5'000-1:10'000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
Crossreactivity	Human
Specificity	Recognizes human adiponectin. Detects a band of ~30kDa by Western blot. Cross-reactivity with other species not tested.
Purity Detail	Protein G-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Mouse IgG1 Isotype Control
RRID	AB_2490083
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels.

Product References

Plasma resistin concentrations measured by enzyme-linked immunosorbent assay using a newly developed monoclonal antibody are elevated in individuals with type 2 diabetes mellitus: B.S. Youn, et al.; J. Clin. Endocrinol. Metab. 89, 150 (2004)
Genetic association study of adiponectin polymorphisms with risk of Type 2 diabetes mellitus in Korean population: Y.Y. Lee, et al.; Diabet. Med. 22, 569 (2005)
Adiponectin expression in human fetal tissues during mid- and late gestation: S. Corbetta, et al,; J. Clin. Endocrinol. Metab. 90, 2397(2005)
Renin receptor expression in human adipose tissue: V. Achard, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 292, R274 (2007)
Adiponectin immunohistochemical expression in colorectal cancer and its correlation with histological grade and tumour microvessel density: V. Barresi, et al.; Pathology 41, 533 (2009)
The expression of adiponectin receptors Adipo-R1 and Adipo-R2 is associated with an intestinal histotype and longer survival in gastric carcinoma: V. Barresi, et al.; J. Clin. Pathol. 62, 705 (2009)
Effect of monomeric adiponectin on cardiac function and perfusion in anesthetized pig: E. Grossini, et al.; J. Endocrinol. 222, 137 (2014)
Acute vitamin D3 supplementation in severe obesity: Evaluation of multimeric adiponectin: S. Mai, et al.; Nutrients 9, 459 (2017)

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