anti-Adiponectin (mouse), mAb (MADI 1147)
AG-20A-0003-C05050 µgCHF 270.00
AG-20A-0003-C100100 µgCHF 350.00
Western Blot analysis of adiponectin in mouse, human and rat plasma using anti-Adiponectin (mouse), mAb (MADI 1147) (Prod. No. AG-20A-0003) at 0.2μg/ml.
|Synonyms||ACRP30; AdipoQ; apM1; GBP28; Adipocyte Complement Related Protein of 30kDa|
|Product Type||Monoclonal Antibody|
|Immunogen/Antigen||Recombinant mouse adiponectin.|
ELISA: (direct and indirect: 1:5’000-1:10’000).
Immunoprecipitation: (1-5μg per sample in a final reaction volume of 500-1000μl).
Western blot: (1:5’000-1:10’000 using ECL; suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
|Specificity||Recognizes mouse adiponectin. Detects a band of ~30kDa by Western blot. Weakly cross-reacts with human and rat adiponectin.|
|Purity Detail||Protein G-affinity purified.|
|Formulation||Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
|Use/Stability||Stable for at least 1 year after receipt when stored at -20°C.|
|Product Specification Sheet|
ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels.
- Adverse effects of the classic antioxidant uric acid in adipocytes: NADPH oxidase-mediated oxidative/nitrosative stress: Y.Y. Sautin, et al.; Am. J. Physiol. Cell. Physiol. 293, C584 (2007)