AdipoGen Life Sciences

anti-FGF-19 (human), mAb (FG369-1)

CHF 190.00

In stock

AG-20A-0066-C05050 µgCHF 190.00

AG-20A-0066-C100100 µgCHF 320.00

Figure 1: Immunohistochemical staining of FGF-19 using anti-FGF-19 (human), mAb (FG369-1) (Prod. No. AG-20A-0066) in formalin-fixed and paraffin-embedded (FFPE) human kidney tissue (15µg/ml).

Figure 2: Western blot analysis using anti-FGF-19 (human), mAb (FG369-1) (Prod. No. AG-20A-0066) at 1:2000 dilution.
1: Human FGF-19 (His-tagged).
2: Mouse vaspin (His-tagged) (negative control).

Specifications / Handling

More Information
Product Details
Synonyms	Fibroblast Growth Factor 19
Product Type	Monoclonal Antibody
Properties
Clone	FG369-1
Isotype	Mouse IgG1κ
Immunogen/Antigen	Recombinant human FGF-19.
Application	ELISA: (direct or indirect: 1:2'000-1:5'000) Immunohistochemistry: (paraffin sections, 15µg/ml) Western blot: (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application. Note: Tested on recombinant proteins and/or target-protein transfected cell lines in ELISA, Western Blot and/or FACS.
Crossreactivity	Human
Specificity	Recognizes human FGF-19. Detects a band of ~25kDa by Western blot.
Purity Detail	Protein G-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Mouse IgG1 Isotype Control
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

FGF-19 is involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. FGF-19 plays an important role in brain development during embryogenesis. FGF-19 binds only to FGF receptor 4 (FGFR4). Activity requires the presence of KLB.

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