anti-NLRP3/NALP3 (mouse), mAb (Cryo-1)

CHF 520.00
In stock
AG-20B-0006-C100100 µgCHF 520.00
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Product Details
Synonyms NACHT-, LRR- and PYD-containing Protein 3; NLRP3; Cryopyrin; Cold Autoinflammatory Syndrome 1 Protein
Product Type Monoclonal Antibody
Properties
Clone Cryo-1
Isotype Mouse IgG2b
Source/Host Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen Recombinant mouse NLRP3/NALP3 (pyrin domain/aa 1-93).
Application

Western Blot (see online protocol): (1μg/ml)

Crossreactivity Mouse
Specificity

Recognizes mouse NLRP3/NALP3.

Purity ≥95% (SDS-PAGE)
Purity Detail Protein G-affinity purified.
Concentration 1mg/ml
Formulation Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Isotype Negative Control

Mouse IgG2b Isotype Control

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
Protocols Download PDF
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

The inflammasome is a multiprotein complex that mediates the activation of caspase-1, which promotes, amongst others, the secretion of the proinflammatory cytokines interleukin (IL)-1β and IL-18. Members of the Nod-like receptor family, including NLRP3/NALP3, are critical components of the inflammasome that link danger-signals to caspase-1 activation. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1), Muckle-Wells syndrome (MWS) and of chronic infantile neurologic cutaneous and articular syndrome (CINCA).

Product References
  1. Nlrp6 promotes recovery after peripheral nerve injury independently of inflammasomes: E. Ydens, et al.; J. Neuroinfl. 12, 143 (2015)
  2. A Novel Role for Brain Natriuretic Peptide: Inhibition of IL-1β Secretion via Downregulation of NF-kB/Erk 1/2 and NALP3/ASC/Caspase-1 Activation in Human THP-1 Monocyte: L. Mezzasoma, et al.; Mediators Inflamm. (2017)
  3. Complement Mediated Activation of the NLRP3 Inflammasome and its Inhibition by AAV Mediated Delivery of CD59 in a Model of Uveitis: B. Kumar, et al.; Mol. Ther. (2018), doi: 10.1016/j.ymthe.2018.03.012
  4. Selective inhibition of the p38α MAPK–MK2 axis inhibits inflammatory cues including inflammasome priming signals: C. Wang, et al.; J. Exp. Med. 215, (2018)
  5. Gut Microbes Egested During Bites of Infected Sand flies Augment Severity of Leishmaniasis Via Inflammasome-Derived IL-1β: R. Dey, et al.; Cell Host Microbe. 23, 134 (2018)
  6. Repositioning of the β-Blocker Carvedilol as a Novel Autophagy Inducer That Inhibits the NLRP3 Inflammasome: WT. Wong, et al.; Front. Immunol. 9, 1920 (2018)
  7. Anti-Inflammatory (M2) Response Is Induced by a sp2-Iminosugar Glycolipid Sulfoxide in Diabetic Retinopathy: F. Cano-Cano, et al.; Front. Immunol. 12, 632132 (2021)
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