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AdipoGen Life Sciences
anti-NLRP3/NALP3 (mouse), mAb (Cryo-1)
520
CHF
CHF 520.00
In stock
AG-20B-0006-C100100 µgCHF 520.00
Figure 1: NLRP3 is detected in mouse macrophages using anti-NLRP3/NALP3 (mouse), mAb (Cryo-1) (Prod. No AG-20B-0006).
Cell extracts from mouse macrophages, WT (lane 1) or NLRP3 KO (lane 2), were separated by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with anti-NLRP3/NALP3 (mouse), mAb (Cryo-1) (1μg/ml). Proteins were visualized by a chemiluminescence detection system.
Cell extracts from mouse macrophages, WT (lane 1) or NLRP3 KO (lane 2), were separated by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with anti-NLRP3/NALP3 (mouse), mAb (Cryo-1) (1μg/ml). Proteins were visualized by a chemiluminescence detection system.
| Product Details | |
|---|---|
| Synonyms | NACHT-, LRR- and PYD-containing Protein 3; NLRP3; Cryopyrin; Cold Autoinflammatory Syndrome 1 Protein |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | Cryo-1 |
| Isotype | Mouse IgG2b |
| Source/Host | Purified from concentrated hybridoma tissue culture supernatant. |
| Immunogen/Antigen | Recombinant mouse NLRP3/NALP3 (pyrin domain/aa 1-93). |
| Application |
Western Blot (see online protocol): (1μg/ml) |
| Crossreactivity | Mouse |
| Specificity |
Recognizes mouse NLRP3/NALP3. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| Protocols |
 Download PDF |
| MSDS |
Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The inflammasome is a multiprotein complex that mediates the activation of caspase-1, which promotes, amongst others, the secretion of the proinflammatory cytokines interleukin (IL)-1β and IL-18. Members of the Nod-like receptor family, including NLRP3/NALP3, are critical components of the inflammasome that link danger-signals to caspase-1 activation. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1), Muckle-Wells syndrome (MWS) and of chronic infantile neurologic cutaneous and articular syndrome (CINCA).
Product References
- Nlrp6 promotes recovery after peripheral nerve injury independently of inflammasomes: E. Ydens, et al.; J. Neuroinfl. 12, 143 (2015)
- A Novel Role for Brain Natriuretic Peptide: Inhibition of IL-1β Secretion via Downregulation of NF-kB/Erk 1/2 and NALP3/ASC/Caspase-1 Activation in Human THP-1 Monocyte: L. Mezzasoma, et al.; Mediators Inflamm. (2017)
- Complement Mediated Activation of the NLRP3 Inflammasome and its Inhibition by AAV Mediated Delivery of CD59 in a Model of Uveitis: B. Kumar, et al.; Mol. Ther. (2018), doi: 10.1016/j.ymthe.2018.03.012
- Selective inhibition of the p38α MAPK–MK2 axis inhibits inflammatory cues including inflammasome priming signals: C. Wang, et al.; J. Exp. Med. 215, (2018)
- Gut Microbes Egested During Bites of Infected Sand flies Augment Severity of Leishmaniasis Via Inflammasome-Derived IL-1β: R. Dey, et al.; Cell Host Microbe. 23, 134 (2018)
- Repositioning of the β-Blocker Carvedilol as a Novel Autophagy Inducer That Inhibits the NLRP3 Inflammasome: WT. Wong, et al.; Front. Immunol. 9, 1920 (2018)
- Anti-Inflammatory (M2) Response Is Induced by a sp2-Iminosugar Glycolipid Sulfoxide in Diabetic Retinopathy: F. Cano-Cano, et al.; Front. Immunol. 12, 632132 (2021)
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