AdipoGen Life Sciences

anti-ZBP1, mAb (Zippy-1)

CHF 495.00
In stock
AG-20B-0010-C100100 µgCHF 495.00
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Product Details
Synonyms Z-DNA-binding Protein 1; Tumor Stroma and Activated Macrophage Protein DLM-1; DNA-dependent Activator of IFN-regulatory Factors; DAI
Product Type Monoclonal Antibody
Clone Zippy-1
Isotype Mouse IgG2a
Source/Host Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen Recombinant mouse ZBP1 (aa 1-411).

Immunocytochemistry: (1:500) (see reference 1)
Immunoprecipitation: (1:200)
Western Blot: (1:1’000)

Crossreactivity Human

Recognizes human and mouse ZBP1.

Purity ≥95% (SDS-PAGE)
Purity Detail Protein G-affinity purified.
Concentration 1mg/ml
Formulation Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Isotype Negative Control

Mouse IgG2a Isotype Control

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
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Product Specification Sheet
Datasheet Download PDF

ZBP1 is an interferon inducible protein that binds to cytoplasmic DNA and plays a role in host defense against tumors and pathogens. ZBP1 is highly expressed in lymphatic tissues including lymph node, leukocytes, tonsil, bone marrow and spleen. ZBP1 is an innate immune sensor of Influenza A virus (IAV). ZBP1 regulates NLRP3 (nucleotide and oligomerization domain, leucine-rich repeat-containing protein family, pyrin domain containing 3) inflammasome activation as well as induction of different types of cell death (apoptosis, necroptosis and pyroptosis) during IAV infection.

Product References
  1. ZBP1/DAI is an innate sensor of influenza virus triggering the NLRP3 inflammasome and programmed cell death pathways: T. Kuriakose, et al.; Sci. Immunol. 1, aag2045 (2016)
  2. DAI senses Influenza A virus genomic RNA and activates RIPK3-dependent cell death: R.J. Thapa, et al.; Cell Host & Microbe 20, 674 (2016)
  3. RIPK1 counteracts ZBP1-mediated necroptosis to inhibit inflammation: J. Lin, et al.; Nature 540, 124 (2016)
  4. RIPK1 inhibits ZBP1-driven necroptosis during development: K. Newton, et al.; Nature 540, 129 (2016)
  5. IRF1 Is a Transcriptional Regulator of ZBP1 Promoting NLRP3 Inflammasome Activation and Cell Death during Influenza Virus Infection: T. Kuriakose, et al.; J. Immunol. 200, 1489 (2018)
  6. Necroptotic signaling is primed in Mycobacterium tuberculosis-infected macrophages, but its pathophysiological consequence in disease is restricted: M.D. Stutz, et al.; Cell Death Differ. 25, 951 (2018)
  7. Species-independent contribution of ZBP1/DAI/DLM-1-triggered necroptosis in host defense against HSV1: H. Guo, et al.; Cell Death Dis. 9, 816 (2018) [KO Validation]
  8. Constitutive interferon signaling maintains critical threshold of MLKL expression to license necroptosis: J. Sarhan, et al.; Cell Death Diff. 26, 332 (2019)
  9. ZBP1/DAI Drives RIPK3-Mediated Cell Death Induced by IFNs in the Absence of RIPK1: J.P. Ingram, et al.; J. Immunol. 203, 1348 (2019)
  10. IRF3 and IRF7 contribute to diesel exhaust particles-induced pulmonary inflammation by mediating mTORC1 activation and restraining autophagy in mice: C. Wang, et al.; Eur. J. Immunol. 50, 1142 (2020)
  11. Influenza Virus Z-RNAs Induce ZBP1-Mediated Necroptosis: T. Zhang, et al.; Cell 180, 1115 (2020)
  12. Z-nucleic-acid sensing triggers ZBP1-dependent necroptosis and inflammation: H. Jiao, et al.; Nature 580, 391 (2020)
  13. Caspase-6 Is a Key Regulator of Innate Immunity, Inflammasome Activation, and Host Defense: M. Zheng, et al.; Cell 181, 674 (2020)
  14. Identification of the PANoptosome: A Molecular Platform Triggering Pyroptosis, Apoptosis, and Necroptosis (PANoptosis): S. Christgen, et al.; Front. Cell.Infect. Microbiol. 10, 237 (2020)
  15. ZBP1 promotes fungi-induced inflammasome activation and pyroptosis, apoptosis, and necroptosis (PANoptosis): B. Banoth, et al.; J. Biol. Chem. 295, P18276 (2020)
  16. ZBP1 promotes LPS-induced cell death and IL-1β release via RHIM-mediated interactions with RIPK1: H.I. Muendlein, et al.; Nat. Commun. 12, 86 (2021)
  17. Vaccinia virus E3 prevents sensing of Z-RNA to block ZBP1-dependent necroptosis: H. Koehler, et al.; Cell Host Microbe 29, 1 (2021)
  18. ZBP1 not RIPK1 mediates tumor necroptosis in breast cancer: J.Y. Baik, et al.; Nature Commun. 12, 2666 (2021)
  19. Loss of Mitochondrial Protease CLPP Activates Type I IFN Responses through the Mitochondrial DNA-cGAS-STING Signaling Axis: S. Torres-Odio, et al.; J. Immunol. 206, 1890 (2021)
  20. ADAR1 restricts ZBP1-mediated immune response and PANoptosis to promote tumorigenesis: R. Karki, et al.; Cell Rep. 37, 109858 (2021)
  21. Z-DNA binding protein 1 mediates necroptotic and apoptotic cell death pathways in murine astrocytes following herpes simplex virus-1 infection: A.M. Jeffries, et al.; J. Neuroinflamm. 19, 109 (2022)
  22. ADAR1 mutation causes ZBP1-dependent immunopathology: N.W. Hubbard, et al.; Nature 607, 769 (2022)
  23. ADAR1 averts fatal type I interferon induction by ZBP1: H. Jiao, et al.; Nature 607, 776 (2022)
  24. ADAR1 prevents autoinflammation by suppressing spontaneous ZBP1 activation: R. De Reuver, et al.; Nature 607, 784 (2022)
  25. ZBP1-dependent inflammatory cell death, PANoptosis, and cytokine storm disrupt IFN therapeutic efficacy during coronavirus infection: R. Karki, et al.; Sci. Immunol. 7, eabo6294 (2022)
  26. Necroptosis of macrophage is a key pathological feature in biliary atresia via GDCA/S1PR2/ZBP1/p-MLKL axis: S. Yang, et al. Nature Cell Death Dis. 14, 175 (2023)
  27. Polyamine metabolism controls B-to-Z DNA transition to orchestrate DNA sensor cGAS activity: Ch. Zhao, et al.; Immunity 56, (2023)
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