AdipoGen Life Sciences

anti-BAFF-R (human), mAb (HuBR9.1) (ATTO 488)

CHF 450.00

In stock

AG-20B-0016TD-T100100 testsCHF 450.00

Figure 1: Detection of endogenous human BAFF-R with anti-BAFF-R (human), mAb (HuBR9.1) (AG-20B-0016).
BJAB and Jurkat cells were stained with A) 20 μg /ml (and 2-fold dilutions) and B) 2μg /ml of anti-BAFF-R (human), mAb (HuBR9.1) in 50 μl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide), revealed with anti-mouse-PE (1/200) and then analyzed by flow cytometry.

Specifications / Handling

More Information
Product Details
Synonyms	BAFF Receptor; BR3; BlySR3; CD268; TNFRSF13C
Product Type	Monoclonal Antibody
Properties
Clone	HuBR9.1
Isotype	Mouse IgG1
Source/Host	Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen	Cells transfected with human BAFF-R.
Label/Conjugates	ATTO 488
Application	Flow Cytometry Definition for 1 test: 1μl of labeled antibody in 200μl of Flow Cytometry buffer (see online protocol).
Crossreactivity	Human
Specificity	Recognizes human BAFF-R.
Purity	≥95% (SDS-PAGE)
Purity Detail	Protein G-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. In PBS containing 0.02% sodium azide.
Isotype Negative Control	Mouse IgG1 Isotype Control (ATTO 488)
Other Product Data	New ATTO-fluorescent antibodies show increased photostability, outstanding brightness and intense signals. ATTO dyes are thermally stable, resistant to environmental changes and show no significant isomerization. ATTO 488 shows bright green fluorescence (λabs (max): 501nm, λem (max): 523nm, εmax: 90’000).
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	+4°C
Handling Advice	Do not freeze. Protect from light.
Use/Stability	Keep conjugated formats at +4°C. Stable for at least 1 year after receipt when stored at +4°C.
Documents
Protocols	Download PDF
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Three receptors for BAFF were recently identified and designated TACI, BCMA and BAFF-R. BAFF-R signaling is essential for B cell development, survival and humoral immune responses.

Product References

BAFF-R expression correlates with positive selection of immature B cells: R. Tussiwand, et al.; Eur. J. Immunol. 42, 206 (2012)
A loop region of BAFF controls B cell survival and regulates recognition by different inhibitors: M. Vigolo, et al.; Nat. Commun. 9, 1199 (2018)

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