BAFF-R (human):Fc (human) (rec.)
|Synonyms||TNFRSF 13C; Tumor Necrosis Factor Receptor Superfamily Member 13C; BR3; BlySR3; CD268; BAFF Receptor|
|Source/Host||HEK 293 cells|
The extracellular domain of human BAFF-R (aa 2-71) is fused at the C-terminus to the Fc portion of human IgG1.
Binds to human and mouse BAFF.
Inhibits BAFF activity. Detection of membrane-bound human and mouse BAFF in combination with PAb to human IgG1.
|Endotoxin Content||<0.01EU/μg purified protein (LAL test; Lonza).|
|Concentration||1mg/ml after reconstitution.|
|Reconstitution||Reconstitute with 50μl sterile water.|
|Formulation||Lyophilized. Contains PBS.|
|Protein Negative Control|
|Other Product Data||
UniProt link Q96RJ3: BAFF-R (human)
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
|Product Specification Sheet|
BAFF is mainly produced by innate immune cells such as neutrophils, monocytes, macrophages, dendritic cells, follicular dendritic cells. T cells, activated B cells, some malignant B cells and also non-lymphoid cells like astrocytes, synoviocytes and epithelial cells can also produce BAFF. BAFF binds three distinct receptors (BAFF-R, TACI and BCMA) expressed predominantly on B cells, although activated T cells also express BAFF-R. BAFF is a master regulator of peripheral B cell survival, and together with IL-6, promotes Ig class-switching and plasma cell differentiation . Besides its major role in B cell biology, BAFF co-stimulates activated T cells. Deregulated expression of BAFF leads to autoimmune disorders in mice. In humans, elevated levels of soluble BAFF have been detected in the serum of patients with various autoimmune diseases, such as Sjögren’s syndrome , Rheumatoid Arthritis (RA) , Multiple sclerosis (MS) and Systemic Lupus Erythematosus (SLE) . BAFF is also increased levels in some lymphoid cancers.
- No evidence that soluble TACI induces signalling via membrane-expressed BAFF and APRIL in myeloid cells: J. Nys, et al.; PLoS One 8, e61350 (2013)
- A loop region of BAFF controls B cell survival and regulates recognition by different inhibitors: M. Vigolo, et al.; Nat. Commun. 9, 1199 (2018)