anti-BAFF (mouse), mAb (blocking) (Sandy-2) (preservative free)

CHF 470.00
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AG-20B-0063PF-C100100 µgCHF 470.00
AG-20B-0063PF-C500500 µgCHF 1'100.00
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Product Details
Synonyms BLyS; TALL-1; CD257; B Cell Activating Factor; TNFSF13B
Product Type Monoclonal Antibody
Properties
Clone Sandy-2
Isotype Mouse IgG1κ
Source/Host Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen Recombinant mouse BAFF.
Label/Conjugates Preservative Free
Application

Functional Application: Inhibition of mouse BAFF binding to BAFF-R and TACI (BCMA not tested); blocks BAFF activity in mice. Injection of the anti-BAFF (mouse), mAb (Sandy-2) in mice creates a BAFF KO phenotype within 2 weeks, that can be maintained as long as necessary (> 6 months) by further injections of Sandy-2 every 2 weeks.
Immunoprecipitation: (1:200)

Crossreactivity Mouse
Specificity

Recognizes mouse BAFF.

Purity ≥95% (SDS-PAGE)
Purity Detail Protein G-affinity purified.
Endotoxin Content <0.01EU/μg purified protein (LAL test; Lonza).
Concentration 1mg/ml
Formulation Liquid. In PBS.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice Do not freeze.
Use/Stability Stable for at least 1 year after receipt when stored at +4°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

BAFF is a master regulator of peripheral B cell survival and, together with IL-6, promotes Ig class-switching and plasma cell differentiation. BAFF co-stimulates activated T cells. Increased levels of soluble BAFF have been detected in the serum of patients with various autoimmune diseases, such as Sjögren’s syndrome, rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus (SLE). Furthermore, BAFF is found in inflammatory sites in which there is lymphoid neogenesis. BAFF levels are elevated in patients with multiple myeloma and B cell chronic lymphoid leukemia (B-CCL).

Product References
  1. The B cell-stimulatory cytokines BLyS and APRIL are elevated in human periodontitis and are required for B cell-dependent bone loss in experimental murine periodontitis: T. Abe, et al.; J. Immunol. 195, 1427 (2015)
  2. Antibodies that block or activate mouse B cell activating factor of the TNF family (BAFF) respectively induce B cell depletion or B cell hyperplasia: C. Kowalczyk-Quintas, et al.; J. Biol. Chem. 291, 19826 (2016)
  3. Interactions between fibroblastic reticular cells and B cells promote mesenteric lymph node lymphangiogenesis: L.K. Dubey, et al.; Nat. Commun. 8, 367 (2017)
  4. The unknown aspect of BAFF: Inducing IL-35 production by a CD5+CD1dhiFcγRIIbhi regulatory B-Cell subset in lupus: Y. Zhang, et al.; J. Invest. Dermatol. 137, 2532 (2017)
  5. Berberine demonstrates anti-inflammatory properties in Helicobacter pylori-infected mice with chronic gastritis by attenuating the Th17 response triggered by the B cell-activating factor: X. Wu, et al.; J. Cell. Biochem. 119, 5373 (2018)
  6. A loop region of BAFF controls B cell survival and regulates recognition by different inhibitors: M. Vigolo, et al.; Nat. Commun. 9, 1199 (2018)
  7. Healthy donor polyclonal IgMs diminish B-lymphocyte autoreactivity, enhance regulatory T cell generation, and reverse type 1 diabetes in NOD mice: C.S. Wilson, et al.; Diabetes 67, 2349 (2018)
  8. B2-Lymphocyte responses to oxidative stress-derived antigens contribute to the evolution of nonalcoholic fatty liver disease (NAFLD): S. Bruzzi, et al.; Free. Radic. Biol. Med. 124, 249 (2018)
  9. Depletion of BAFF cytokine exacerbates infection in Pseudomonas aeruginosa infected mice: D. Garic, et al.; J. Cyst. Fibr. 18, 349 (2019)
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