BAFF, Soluble (mouse) (rec.)

CHF 260.00
In stock
AG-40B-0022-C01010 µgCHF 260.00
AG-40B-0022-30103 x 10 µgCHF 520.00
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Product Details
Synonyms Tumor Necrosis Factor Ligand Superfamily Member 13B; TNFSF13B; B Cell Activating Factor; CD257
Product Type Protein
Properties
Source/Host HEK 293 cells
Sequence Mouse BAFF (aa 127-309) is fused at the N-terminus to a FLAG®-tag.
Crossreactivity Human
Mouse
Specificity Binds to human and mouse BAFF-R, TACI and BCMA.
MW ~26kDa (SDS-PAGE)
Purity ≥90% (SDS-PAGE)
Endotoxin Content <0.01EU/μg purified protein (LAL test; Lonza).
Concentration 0.1mg/ml after reconstitution.
Reconstitution Reconstitute with 100μl sterile water.
Formulation Lyophilized. Contains PBS.
Other Product Data

Uniprot link Q9WU72: BAFF (mouse)

AfCS Signalling Gateway link A000383: BAFF (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

BAFF is mainly produced by innate immune cells such as neutrophils, monocytes, macrophages, dendritic cells, follicular dendritic cells. T cells, activated B cells, some malignant B cells and also non-lymphoid cells like astrocytes, synoviocytes and epithelial cells can also produce BAFF. BAFF binds three distinct receptors (BAFF-R, TACI and BCMA) expressed predominantly on B cells, although activated T cells also express BAFF-R. BAFF is a master regulator of peripheral B cell survival, and together with IL-6, promotes Ig class-switching and plasma cell differentiation. Besides its major role in B cell biology, BAFF co-stimulates activated T cells. Deregulated expression of BAFF leads to autoimmune disorders in mice. In humans, elevated levels of soluble BAFF have been detected in the serum of patients with various autoimmune diseases such as Sjoegren syndrome, Rheumatoid arthritis (RA), Multiple sclerosis (MS) and Systemic Lupus Erythematosus (SLE). BAFF has also increased levels in some lymphoid cancers.

Product References
  1. Selective APRIL blockade delays systemic lupus erythematosus in mouse: B. Huard, et al.; PLoS One 7, e31837 (2012)
  2. B Cell–Intrinsic mTORC1 Promotes Germinal Center–Defining Transcription Factor Gene Expression, Somatic Hypermutation, and Memory B Cell Generation in Humoral Immunity: A.L. Raybuck, et al.; J. Immunol. 200, (2018)
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