Fc (human):BAFF (human) (rec.)

CHF 290.00
In stock
AG-40B-0120-C01010 µgCHF 290.00
AG-40B-0120-30103 x 10 µgCHF 580.00
AG-40B-0120-C500500 µgCHF 2'900.00
More Information
Product Details
Synonyms BLyS; TALL-1; CD257; B Cell Activating Factor; TNFSF13B; Tumor Necrosis Factor Ligand Superfamily Member 13B
Product Type Protein
Properties
Source/Host CHO cells
Sequence

The receptor-binding domain of human BAFF (aa 136-285) is fused at the N-terminus to the Fc portion of human IgG1.

Crossreactivity Human
Mouse
Specificity

Binds to human and mouse BAFF-R, TACI and BCMA.

Biological Activity

Rescues the production of mature follicular and marginal zone B cells.
- in vitro: Addition of 1-100 ng/ml of Fc-BAFF induces B cell survival in a dose-dependent manner.
- in vivo: Fc-BAFF treatment rescues mature B cell development in BAFF deficient mice. Mice are injected at day 0 with 100 µg Fc-BAFF i.v. and at day 14 with 50 µg Fc-BAFF i.p.

MW ~50kDa (SDS-PAGE; reducing conditions)
~100kDa (SDS-PAGE; non-reducing conditions)
~300kDa (SDS-PAGE; native conditions; multimerizes and forms hexamers)
Purity ≥95% (SDS-PAGE)
Purity Detail Protein A affinity purified.
Endotoxin Content <0.01EU/μg purified protein (LAL test; Lonza).
Concentration After reconstitution:
for 10µg size: 0.1mg/ml
for 500µg size: 1 mg/ml
Reconstitution 10µg size: Reconstitute with 100µl sterile water.
500µg size: Reconstitute with 500µl sterile water.
Formulation Lyophilized. Contains PBS.
Protein Negative Control

Fc (human) IgG1 Control (rec.)

Other Product Data

UniProt link Q9Y275: BAFF (human)

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

BAFF is mainly produced by innate immune cells such as neutrophils, monocytes, macrophages, dendritic cells, follicular dendritic cells. T cells, activated B cells, some malignant B cells and also non-lymphoid cells like astrocytes, synoviocytes and epithelial cells can also produce BAFF. BAFF binds three distinct receptors (BAFF-R, TACI and BCMA) expressed predominantly on B cells, although activated T cells also express BAFF-R. BAFF is a master regulator of peripheral B cell survival, and together with IL-6, promotes Ig class-switching and plasma cell differentiation. Besides its major role in B cell biology, BAFF co-stimulates activated T cells. Deregulated expression of BAFF leads to autoimmune disorders in mice. In humans, elevated levels of soluble BAFF have been detected in the serum of patients with various autoimmune diseases such as Sjoegren syndrome, Rheumatoid arthritis (RA), Multiple sclerosis (MS) and Systemic Lupus Erythematosus (SLE). BAFF has also increased levels in some lymphoid cancers.

Product References
  1. Rescue of the mature B cell compartment in BAFF-deficient mice by treatment with recombinant Fc-BAFF: L.K. Swee, et al.; Immunol. Lett. 131, 40 (2010)
  2. Soluble BAFF levels inversely correlate with peripheral B cell numbers and the expression of BAFF receptors: M. Kreuzaler, et al.; J. Immunol. 188, 497 (2012)
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