AdipoGen Life Sciences

BAFF, Soluble (human) (60-mer) (rec.) (highly active)

CHF 290.00
In stock
AG-40B-0112-C01010 µgCHF 290.00
AG-40B-0112-30103 x 10 µgCHF 580.00
More Information
Product Details
Synonyms BLyS; TALL-1; CD257; B Cell Activating Factor; TNFSF13B; Tumor Necrosis Factor Ligand Superfamily Member 13B
Product Type Protein
Properties
Source/Host E. coli
Sequence

The extracellular domain of human BAFF (aa 134-285) is fused at the N-terminus to a His-tag.

Crossreactivity Human
Mouse
Specificity

Binds to human and mouse BAFF-R, TACI and BCMA. Relative binding affinity (EC50) for BAFF-R (human): ~3ng/ml.

Biological Activity

Increases B cell survival/proliferation. Increases CD21/CD23 expression on B cells in vivo. Activates BAFF-R, TACI and BCMA receptors. Works at concentrations <20ng/ml.

MW ~19kDa (under reduced conditions)
~1100kDa (under native conditions)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.01EU/μg purified protein (LAL test; Lonza).
Concentration 0.1mg/ml after reconstitution.
Reconstitution Reconstitute with 100μl sterile water.
Formulation Lyophilized. Contains PBS.
Other Product Data

UniProt link Q9Y275: BAFF (human)

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

BAFF is mainly produced by innate immune cells such as neutrophils, monocytes, macrophages, dendritic cells, follicular dendritic cells. T cells, activated B cells, some malignant B cells and also non-lymphoid cells like astrocytes, synoviocytes and epithelial cells can also produce BAFF. BAFF binds three distinct receptors (BAFF-R, TACI and BCMA) expressed predominantly on B cells, although activated T cells also express BAFF-R. BAFF is a master regulator of peripheral B cell survival, and together with IL-6, promotes Ig class-switching and plasma cell differentiation. Besides its major role in B cell biology, BAFF co-stimulates activated T cells. Deregulated expression of BAFF leads to autoimmune disorders in mice. In humans, elevated levels of soluble BAFF have been detected in the serum of patients with various autoimmune diseases such as Sjoegren syndrome, Rheumatoid arthritis (RA), Multiple sclerosis (MS) and Systemic Lupus Erythematosus (SLE). BAFF has also increased levels in some lymphoid cancers.

Processed human BAFF can either remain as a trimer, which is usual for TNF family ligands or assemble into 60-mer composed of 20 trimers. Mouse BAFF 60-mer has been identified in the serum of BAFF transgenic mice. Oligomerization of BAFF 3-mer into 60-mer in human BAFF is prevented by mutation of His218, a residue critical for 3-mer-to-3-mer interactions, but not for receptor binding. Despite the predominant functional role of processed BAFF in vivo, membrane-bound BAFF might also play a role. Indeed, soluble BAFF (3-mer) can trigger BAFF-R but not TACI or BCMA, whereas oligomeric forms of BAFF (BAFF 60-mer), which mimic membrane-bound BAFF, activate all BAFF receptors.

Product References
  1. TACI, unlike BAFF-R, is solely activated by oligomeric BAFF and APRIL to support survival of activated B cells and plasmablasts: C. Bossen, et al.; Blood 111, 1004 (2008)
  2. Mutation of the BAFF furin cleavage site impairs B-cell homeostasis and antibody responses: C. Bossen, et al.; Eur. J. Immunol. 41, 787 (2011)
  3. No evidence that soluble TACI induces signalling via membrane-expressed BAFF and APRIL in myeloid cells: J. Nys, et al.; PLoS One 8, e61350 (2013)
  4. BAFF 60-mer, and differential BAFF 60-mer dissociating activities in human serum, cord blood and cerebrospinal fluid: M. Eslamy, et al.; Front. Cell Dev. Biol. 8, 577662 (2020)
  5. APRIL limits atherosclerosis by binding to heparan sulfate proteoglycans: D. Tsiantoulas, et al.; Nature 597, 92 (2021)
  6. Ligand-independent oligomerization of TACI is controlled by the transmembrane domain and regulates proliferation of activated B cells: C.R. Smulski, et al.; Cell Rep. 38, 110583 (2022)
  7. BAFF antagonism via the BAFF receptor 3 binding site attenuates BAFF 60-mer-induced classical NF-κB signaling and metabolic reprogramming of B cells: M. Lempicki, et al.; Cell. Immunol. 381, 104603 (2022)
  8. Povetacicept, an Enhanced Dual APRIL/BAFF Antagonist that Modulates B Lymphocytes and Pathogenic Autoantibodies for the Treatment of Lupus and Other B Cell-Related Autoimmune Diseases: L.S. Evans, et al.; Arthritis Rheumatol. 75, 1187 (2023)
  9. Non-apoptotic FAS signaling controls mTOR activation and extrafollicular maturation in human B cells: J. Staniek, et al.; Sci. Immunol. 9, eadj5948 (2024)
© 2017 Adipogen Life Sciences. Pictures: © 2012 Martin Oeggerli. All Rights Reserved.