anti-Neutrophils (mouse), mAb (blocking) (Nimp-R14)

CHF 330.00
In stock
AG-20B-0043-C100100 µgCHF 330.00
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Product Details
Product Type Monoclonal Antibody
Properties
Clone Nimp-R14
Isotype Rat IgG2b
Source/Host Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen Purified mouse BALB/c neutrophils.
Application

Flow Cytometry:(1:100)
Immunohistochemistry:(1:200; frozen section / paraffin section)
Immunocytochemistry: (1:200)
Functional (Blocking): 250μg / mouse (depletes neutrophils and monocytes in vivo)

Crossreactivity Mouse
Specificity

Recognizes mouse neutrophils (surface protein mouse Ly-6G) and may recognize Ly-6Chigh cells (inflammatory monocytes).

Purity ≥95% (SDS-PAGE)
Purity Detail Protein G-affinity purified.
Endotoxin Content <0.1EU/μg purified protein (LAL test; Lonza).
Concentration 1mg/ml
Formulation Liquid. In PBS containing 0.02% sodium azide.
Isotype Negative Control

Rat IgG2b Isotype Control

Other Product Data

Negative control: AG-35B-0011

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Neutrophils are key components of the inflammatory response and as such contribute to the killing of microorganisms. In addition, recent evidence suggests their involvement in the development of the immune response. The Nimp-R14 mAb allows the depletion of mouse neutrophils and monocytes  in vivo.

Product References
  1. Differentiation antigens on mouse eosinophils and neutrophils identified by monoclonal antibodies: A. Lopez, et al.;  Brit. J. Haematol. 57, 489 (1984)
  2. Schistosoma mansoni: evidence that immunity in vaccinated and chronically infected CBA/Ca mice is sensitive to treatment with a monoclonal antibody that depletes cutaneous effector cells: D. McLaren, et al.;  Parasite Immunol. 9, 667 (1987)
  3. Monocytes/macrophages rather than PMN are involved in early cartilage degradation in cationic immune complex arthritits in mice: P. Van Lent, et al.; J. Leukoc. Biol. 61, 267 (1997)
  4. Adenoviral vector-mediated overexpression of IL-4 in the knee joint of mice with collagen-induced arthritis prevents cartilage destruction: E. Lubberts, et al.; J. Immunol. 163, 4546 (1999)
  5. An immunomodulatory function for neutrophils during the induction of a CD4+ Th2 response in BALB/c mice infected with Leishmania major: F. Tacchini-Cottier, et al.; J. Immunol. 165, 628 (2000)
  6. A new mechanism for IL-5-dependent helminth control: neutrophil accumulation and neutrophil-mediated worm encapsulation in murine filariasis are abolished in the absence of IL-5: K.M. Al-Qaoud, et al.; Int. Immunol. 12, 899 (2000)
  7. Rapid recruitment of inflammatory monocytes is independent of neutrophil migration: R.B. Henderson, et al.; Blood 102, 328 (2003)
  8. Complement factor C5a mediates renal ischemia-reperfusion injury independent from neutrophils: B. de Vries, et al.; J. Immunol. 170, 3883 (2003)
  9. Absence of cross-reactivity between murine Ly-6C and Ly-6G: S. Nagendra, et al.; Cytom. A 58, 195 (2004)
  10. Activation of Toll-Like Receptor (TLR)2, TLR4, and TLR9 in the Mammalian Cornea Induces MyD88-Dependent Corneal Inflammation: A.C. Johnson, et al.;  Invest. Ophthalmol. Vis. Sci. 46, 589 (2005)
  11. Neutrophils contribute to development of a protective immune response during onset of infection with Leishmania donovani: E. McFarlane, et al.; Infect. Immun. 76, 532 (2008)
  12. Innate Immune-induced depletion of bone marrow neutrophils aggravates systemic bacterial infections: A. Navarini, et al.; PNAS 106, 7107 (2009)
  13. Analysis of innate defences against Plasmodium falciparum in immunodeficient mice:  A Ludovic, et al.; Malaria J. 9, 197 (2010)
  14. Role of Neutrophils in the Early Shaping of the Leishmania Major Specific Immune Response in Experimental Murine Cutaneous Leishmaniasis: M. Charmoy, et al.; Neutrophils in Infectious Diseases 1, 49 (2011)
  15. The Influence of Reactive Oxygen Species and Glucocorticoids on Dry Skin in a Mouse Model of Arthritis: K. Goto, et al.; Skin Pharmacol. Physiol. 31, 188 (2018)
  16. Intraperitoneal neutrophils activated by KRAS-induced ovarian cancer exert antitumor effects by modulating adaptive immunity: M. Yoshida, et al.; Int. J. Oncol. 53, 1580 (2018)
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