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AdipoGen Life Sciences
anti-Neutrophils (mouse), mAb (blocking) (Nimp-R14) (preservative free)
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CHF 350.00
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AG-20B-0043PF-C500500 µgCHF 350.00
AG-20B-0043PF-M0022 mgCHF 590.00
AG-20B-0043PF-M01010 mgCHF 1’800.00
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Figure 1: Mouse neutrophils are depleted in vivo by anti-Neutrophils (mouse), mAb (blocking) (Nimp-R14) (preservative free) (AG-20B-0043PF).
Method: Mice were injected) i.p. with 250µg of anti-Neutrophils (mouse), mAb (blocking) (Nimp-R14) (preservative free) (B) or with Control mAb (A) in BALB/c mouse 6 h prior to Leishmania major infection (3x106 parasites injected in the hind footpad). 3 days later, blood (100µl) was subjected to flow cytometric analysis after staining with APC/CY7-labeled anti-Ly6G antibody (clone 1A8).
Method: Mice were injected) i.p. with 250µg of anti-Neutrophils (mouse), mAb (blocking) (Nimp-R14) (preservative free) (B) or with Control mAb (A) in BALB/c mouse 6 h prior to Leishmania major infection (3x106 parasites injected in the hind footpad). 3 days later, blood (100µl) was subjected to flow cytometric analysis after staining with APC/CY7-labeled anti-Ly6G antibody (clone 1A8).
| Product Details | |
|---|---|
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | Nimp-R14 |
| Isotype | Rat IgG2b |
| Source/Host | Purified from concentrated hybridoma tissue culture supernatant. |
| Immunogen/Antigen | Purified mouse BALB/c neutrophils. |
| Label/Conjugates | Preservative Free |
| Application |
Flow Cytometry:(1:100) |
| Crossreactivity | Mouse |
| Specificity |
Recognizes mouse neutrophils (surface protein mouse Ly-6G) and may recognize Ly-6Chigh cells (inflammatory monocytes). |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified. |
| Endotoxin Content | <0.1EU/μg purified protein (LAL test; Lonza). |
| Concentration | Lot dependent |
| Formulation | Liquid. In PBS. |
| Other Product Data |
10mg Size: Delivered as 5 x 2mg vials. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Neutrophils are key components of the inflammatory response and as such contribute to the killing of microorganisms. In addition, recent evidence suggests their involvement in the development of the immune response. The Nimp-R14 mAb allows the depletion of mouse neutrophils and monocytes in vivo.
Product References
- Differentiation antigens on mouse eosinophils and neutrophils identified by monoclonal antibodies: A. Lopez, et al.; Brit. J. Haematol. 57, 489 (1984)
- Schistosoma mansoni: evidence that immunity in vaccinated and chronically infected CBA/Ca mice is sensitive to treatment with a monoclonal antibody that depletes cutaneous effector cells: D. McLaren, et al.; Parasite Immunol. 9, 667 (1987)
- Monocytes/macrophages rather than PMN are involved in early cartilage degradation in cationic immune complex arthritits in mice: P. Van Lent, et al.; J. Leukoc. Biol. 61, 267 (1997)
- Adenoviral vector-mediated overexpression of IL-4 in the knee joint of mice with collagen-induced arthritis prevents cartilage destruction: E. Lubberts, et al.; J. Immunol. 163, 4546 (1999)
- An immunomodulatory function for neutrophils during the induction of a CD4+ Th2 response in BALB/c mice infected with Leishmania major: F. Tacchini-Cottier, et al.; J. Immunol. 165, 628 (2000)
- A new mechanism for IL-5-dependent helminth control: neutrophil accumulation and neutrophil-mediated worm encapsulation in murine filariasis are abolished in the absence of IL-5: K.M. Al-Qaoud, et al.; Int. Immunol. 12, 899 (2000)
- Rapid recruitment of inflammatory monocytes is independent of neutrophil migration: R.B. Henderson, et al.; Blood 102, 328 (2003)
- Complement factor C5a mediates renal ischemia-reperfusion injury independent from neutrophils: B. de Vries, et al.; J. Immunol. 170, 3883 (2003)
- Absence of cross-reactivity between murine Ly-6C and Ly-6G: S. Nagendra, et al.; Cytom. A 58, 195 (2004)
- Activation of Toll-Like Receptor (TLR)2, TLR4, and TLR9 in the Mammalian Cornea Induces MyD88-Dependent Corneal Inflammation: A.C. Johnson, et al.; Invest. Ophthalmol. Vis. Sci. 46, 589 (2005)
- Neutrophils contribute to development of a protective immune response during onset of infection with Leishmania donovani: E. McFarlane, et al.; Infect. Immun. 76, 532 (2008)
- Innate Immune-induced depletion of bone marrow neutrophils aggravates systemic bacterial infections: A. Navarini, et al.; PNAS 106, 7107 (2009)
- Analysis of innate defences against Plasmodium falciparum in immunodeficient mice: A Ludovic, et al.; Malaria J. 9, 197 (2010)
- Role of Neutrophils in the Early Shaping of the Leishmania Major Specific Immune Response in Experimental Murine Cutaneous Leishmaniasis: M. Charmoy, et al.; Neutrophils in Infectious Diseases 1, 49 (2011)
- The Influence of Reactive Oxygen Species and Glucocorticoids on Dry Skin in a Mouse Model of Arthritis: K. Goto, et al.; Skin Pharmacol. Physiol. 31, 188 (2018)
- Intraperitoneal neutrophils activated by KRAS-induced ovarian cancer exert antitumor effects by modulating adaptive immunity: M. Yoshida, et al.; Int. J. Oncol. 53, 1580 (2018)
- Novel protective role for MAP kinase phosphatase 2 in inflammatory arthritis: J. Schroeder, et al.; Rheumat. Musculoskel. Dis. 5, e000711 (2019)
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