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AdipoGen Life Sciences
anti-Notch1 (mouse), mAb (22E5)
320
CHF
CHF 320.00
In stock
AG-20B-0051-C100100 µgCHF 320.00
Figure 1: Detection of endogenous mouse Notch 1 or Notch 2 on resting and activated T cells with anti-Notch1 (mouse), mAb (22E5) (Prod. No. AG-20B-0051) and anti-Notch2, mAb (16F11) (Prod. No. AG-20B-0052), respectively. Method: CD4+ T cells from C57BL/6 mice were treated with anti-CD3 on plastic (solid line), IL-2 (dotted line) or medium alone as a negative control (shaded histogram) for 24h. The staining was revealed with a secondary anti-mouse IgG-PE (1/200) and then analyzed by flow cytometry.
| Product Details | |
|---|---|
| Synonyms | Neurogenic Locus Notch Homolog Protein 1; Motch A; mT14; p300 |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | 22E5 |
| Isotype | Rat IgG2aκ |
| Source/Host | Purified from concentrated hybridoma tissue culture supernatant. |
| Immunogen/Antigen | Recombinant mouse Notch1:Fc (AG-40B-0109). |
| Application |
Flow Cytometry: (1:500) Immunofluorescence: (1:1000) (see Reference 1) |
| Crossreactivity | Mouse |
| Specificity |
Recognizes endogenous mouse Notch-1 receptor. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Notch signaling pathway regulates many different cell fate decisions in both vertebrate and invertebrate species. There are 5 canonical Notch ligands in mammals: Jagged-1, Jagged-2, DLL1, DLL3 and DLL4. These can bind to the four Notch receptors Notch 1-4. It is important for pattern formation during development such as neurogenesis, angiogenesis or myogenesis and regulates T cell development and stem cell maintenance. Notch signaling is also involved in cellular processes through-out adulthood. Signaling via Notch occurs between neighbouring cells and both the receptor and its ligands are transmembrane proteins.
Product References
- Dynamic Regulation of Notch 1 and Notch 2 Surface Expression during T Cell Development and Activation Revealed by Novel Monoclonal Antibodies: E. Fiorini, et al.; J. Immunol.183, 7212 (2009)
- Cutting edge: Thymic NK cells develop independently from T cell precursors: V.S. Ribeiro, et al.; J. Immunol. 185, 4993 (2010)
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