anti-Caspase-4 /11 (p20), mAb (Flamy-1)
|Synonyms||Caspase-11 (mouse); Caspase-4 (human); ICE(rel)-II (human); Protease ICH-2 (human); Protease TX (human); Protease ICH-3 (mouse)|
|Product Type||Monoclonal Antibody|
|Source/Host||Purified from concentrated hybridoma tissue culture supernatant.|
|Immunogen/Antigen||Recombinant mouse Caspase-11 (aa 81-373).|
Western Blot: (1μg/ml)
|Specificity||Recognizes endogenous full-length protein and activated (p20) fragment of mouse and human caspase-4/11. Does not detect human Caspase-5.|
|Purity Detail||Protein G-affinity purified.|
|Formulation||Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
|Use/Stability||Stable for at least 1 year after receipt when stored at -20°C.|
|Product Specification Sheet|
Inflammatory caspases (also known as group I caspases) are encoded by three main genes in humans, caspase-1, caspase-4 and caspase-5 and three main genes in mouse, caspase-1, caspase-11 and caspase-12. Caspase-11 is the murine orthologue of human caspase-4 and -5. Murine caspase-11 is a poorly characterized member of the caspase-1 subfamily. Caspase-11-deficient embryonic fibroblasts are resistant to apoptosis induced by ectopic expression of caspase-1, suggesting that caspase-11 is an upstream activator of caspase-1. Unlike caspase-1, the expression of caspase-11 is LPS-inducible and it is reasonable to postulate that other members of the family are regulated at the transcriptional or translational level by extracellular stimuli.
Recently caspase-11 has been shown to be required for activation of the Nlrp3 inflammasome upon E. coli, V. cholerae and C. rodentium infection. The upstream activators of caspase-11 are TLR4 and TRIF, that modulate enteropathogen-induced inflammasome activation by promoting caspase-11 expression and activation. Formation of ASC foci (specks), a measure of NLRP3/ASC complex formation, requires caspase-11 but not caspase-1 indicating that caspase-11 acts upstream of the NLRP3/ASC complex.
- NLRP3 inflammasome activation downstream of cytoplasmic LPS recognition by both caspase-4 and caspase-5: P.J. Baker, et al.; Eur. J. Immunol. 45, 2918 (2015)
- Generation of Genetic Knockouts in Myeloid Cell Lines Using a Lentiviral CRISPR/Cas9 System: P.J. Baker & S.L. Masters; Methods Mol. Biol. 1714, 41 (2018)