anti-Caspase-4 /11 (p20), mAb (Flamy-1) (Biotin)

CHF 430.00
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AG-20B-0060B-C100100 µgCHF 430.00
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Product Details
Synonyms Caspase-11 (mouse); Caspase-4 (human); ICE(rel)-II (human); Protease ICH-2 (human); Protease TX (human); Protease ICH-3 (mouse)
Product Type Monoclonal Antibody
Clone Flamy-1
Isotype Mouse IgG2bκ
Source/Host Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen Recombinant mouse Caspase-11 (aa 81-373).
Label/Conjugates Biotin
Application Western Blot: (1μg/ml)
Crossreactivity Human
Specificity Recognizes endogenous full-length protein and activated (p20) fragment of mouse and human caspase-4/11. Does not detect human Caspase-5.
Purity ≥95% (SDS-PAGE)
Purity Detail Protein G-affinity purified.
Concentration 1mg/ml
Formulation Liquid. In PBS containing 0.02% sodium azide.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
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Product Specification Sheet
Datasheet Download PDF

Inflammatory caspases (also known as group I caspases) are encoded by three main genes in humans, caspase-1, caspase-4 and caspase-5 and three main genes in mouse, caspase-1, caspase-11 and caspase-12. Caspase-11 is the murine orthologue of human caspase-4 and -5. Murine caspase-11 is a poorly characterized member of the caspase-1 subfamily. Caspase-11-deficient embryonic fibroblasts are resistant to apoptosis induced by ectopic expression of caspase-1, suggesting that caspase-11 is an upstream activator of caspase-1. Unlike caspase-1, the expression of caspase-11 is LPS-inducible and it is reasonable to postulate that other members of the family are regulated at the transcriptional or translational level by extracellular stimuli.
Recently caspase-11 has been shown to be required for activation of the Nlrp3 inflammasome upon E. coli, V. cholerae and C. rodentium infection. The upstream activators of caspase-11 are TLR4 and TRIF, that modulate enteropathogen-induced inflammasome activation by promoting caspase-11 expression and activation. Formation of ASC foci (specks), a measure of NLRP3/ASC complex formation, requires caspase-11 but not caspase-1 indicating that caspase-11 acts upstream of the NLRP3/ASC complex.

Product References
  1. NLRP3 inflammasome activation downstream of cytoplasmic LPS recognition by both caspase-4 and caspase-5: P.J. Baker, et al.; Eur. J. Immunol. 45, 2918 (2015)
  2. Generation of Genetic Knockouts in Myeloid Cell Lines Using a Lentiviral CRISPR/Cas9 System: P.J. Baker & S.L. Masters; Methods Mol. Biol. 1714, 41 (2018)
  3. Guanylate-binding protein 5 licenses caspase11 for Gasdermin-D mediated host resistance to Brucella abortus infection: D.M. Cerqueira, et al.; PLoS Pathogens 14, e1007519 (2018)
  4. Intestinal restriction of Salmonella Typhimurium requires caspase-1 and caspase-11 epithelial intrinsic inflammasomes: S.M. Crowley, et al.; PLoS Pathog. 16, e1008498 (2020)
  5. STING regulates metabolic reprogramming in macrophages via HIF-1α during Brucella infection: M.T.R. Gomes, et al.; PLoS Pathog 17, e1009597 (2021)
  6. NLRP6-caspase 4 inflammasome activation in response to cariogenic bacterial lipoteichoic acid in human dental pulp inflammation: X.X. Tian, et al.; Int. Endodont. J. 54, 916 (2021)
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