anti-PD-1 (mouse), mAb (blocking) (1H10)
Method: Magnetic bead affinity purified CD4+ T cells from C57BL/6 mice are stimulated in vitro with PD-1 (mouse), mAb (blocking) (1H10), anti-CD28 and rat IgG2a isotype (control Ig) (0.25µg/2x105 cells) for 48h. Proliferation is determined by [3H]thymidine incorporation in triplicate wells. The data are representative of five independent experiments. The presence of anti-CD28 mAb increases 1H10 mAb-mediated proliferation.
|Synonyms||CD279; Programmed Cell Death Protein 1|
|Product Type||Monoclonal Antibody|
|Source/Host||Purified from concentrated hybridoma tissue culture supernatant.|
|Immunogen/Antigen||Mouse PD-1 transfected cell line.|
Functional Application: Blocks PD-1 binding. Induces a rapid activation and proliferation of T cells at concentration of 0.25µg/2x105 cells.
Recognizes mouse PD-1 (CD279). Does not cross-react with human PD-1d.
|Purity Detail||Protein G-affinity purified.|
|Endotoxin Content||<0.01EU/μg purified protein (LAL test; Lonza).|
|Formulation||Liquid. In PBS containing 0.02% sodium azide.|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
|Use/Stability||Stable for at least 1 year after receipt when stored at -20°C.|
|Product Specification Sheet|
CD279 (Programmed Cell Death Protein 1; PD-1) is a type I transmembrane protein belonging to the CD28/CTLA-4 family of immunoreceptors that mediate signals for regulating immune responses. Members of the CD28/CTLA-4 family have been shown to either promote T cell activation (CD28 and ICOS) or downregulate T cell activation (CTLA-4 and PD-1). CD279 is expressed on activated T cells, B cells, myeloid cells and on a subset of thymocytes. In vitro, ligation of CD279 inhibits TCR-mediated T cell proliferation and production of IL-1, IL-4, IL-10 and IFN-γ. In addition, CD279 ligation also inhibits BCR mediated signaling. CD279 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases.
- Antibody-mediated signalling through PD-1 costimulates T cells and enhances CD28- dependent proliferation: M.L. del Rio, et al.; Eur. J. Immunol. 35, 3545 (2005)