AdipoGen Life Sciences

anti-PD-1 (mouse), mAb (blocking) (1H10)

CHF 230.00

In stock

AG-20B-0075-C100100 µgCHF 230.00

Figure 1: PD-1 receptor-induced CD4 T cell activation and proliferation by PD-1 (mouse), mAb (blocking) (1H10) (AG-20B-0075).
Method: Magnetic bead affinity purified CD4+ T cells from C57BL/6 mice are stimulated in vitro with PD-1 (mouse), mAb (blocking) (1H10), anti-CD28 and rat IgG2a isotype (control Ig) (0.25µg/2x105 cells) for 48h. Proliferation is determined by [3H]thymidine incorporation in triplicate wells. The data are representative of five independent experiments. The presence of anti-CD28 mAb increases 1H10 mAb-mediated proliferation.

Specifications / Handling

More Information
Product Details
Synonyms	CD279; Programmed Cell Death Protein 1
Product Type	Monoclonal Antibody
Properties
Clone	1H10
Isotype	Rat IgG2aκ
Source/Host	Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen	Mouse PD-1 transfected cell line.
Application	Functional Application: Blocks PD-1 binding. Induces a rapid activation and proliferation of T cells at concentration of 0.25µg/2x10⁵ cells. FACS: (1:200) Optimal conditions must be determined individually for each application.
Crossreactivity	Mouse
Specificity	Recognizes mouse PD-1 (CD279). Does not cross-react with human PD-1d.
Purity	≥95% (SDS-PAGE)
Purity Detail	Protein G-affinity purified.
Endotoxin Content	<0.01EU/μg purified protein (LAL test; Lonza).
Concentration	1mg/ml
Formulation	Liquid. In PBS containing 0.02% sodium azide.
Isotype Negative Control	Rat IgG2a Isotype Control
Accession Number	https://www.ncbi.nlm.nih.gov/protein/NP_005009.2
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

CD279 (Programmed Cell Death Protein 1; PD-1) is a type I transmembrane protein belonging to the CD28/CTLA-4 family of immunoreceptors that mediate signals for regulating immune responses. Members of the CD28/CTLA-4 family have been shown to either promote T cell activation (CD28 and ICOS) or downregulate T cell activation (CTLA-4 and PD-1). CD279 is expressed on activated T cells, B cells, myeloid cells and on a subset of thymocytes. In vitro, ligation of CD279 inhibits TCR-mediated T cell proliferation and production of IL-1, IL-4, IL-10 and IFN-γ. In addition, CD279 ligation also inhibits BCR mediated signaling. CD279 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases.

Product References

Antibody-mediated signalling through PD-1 costimulates T cells and enhances CD28- dependent proliferation: M.L. del Rio, et al.; Eur. J. Immunol. 35, 3545 (2005)
Exosomes secreted by ST3GAL5high cancer cells promote peritoneal dissemination by establishing a premetastatic microenvironment: M. Horie, et al.; Mol. Oncol. ahead of print (2023)

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