anti-PD-1 (mouse), mAb (blocking) (1H10)

CHF 230.00
In stock
AG-20B-0075-C100100 µgCHF 230.00
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Product Details
Synonyms CD279; Programmed Cell Death Protein 1
Product Type Monoclonal Antibody
Properties
Clone 1H10
Isotype Rat IgG2aκ
Source/Host Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen Mouse PD-1 transfected cell line.
Application

Functional Application: Blocks PD-1 binding. Induces a rapid activation and proliferation of T cells at concentration of 0.25µg/2x105 cells.
FACS: (1:200)
Optimal conditions must be determined individually for each application.

Crossreactivity Mouse
Specificity

Recognizes mouse PD-1 (CD279). Does not cross-react with human PD-1d.

Purity ≥95% (SDS-PAGE)
Purity Detail Protein G-affinity purified.
Endotoxin Content <0.01EU/μg purified protein (LAL test; Lonza).
Concentration 1mg/ml
Formulation Liquid. In PBS containing 0.02% sodium azide.
Accession Number https://www.ncbi.nlm.nih.gov/protein/NP_005009.2
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

CD279 (Programmed Cell Death Protein 1; PD-1) is a type I transmembrane protein belonging to the CD28/CTLA-4 family of immunoreceptors that mediate signals for regulating immune responses. Members of the CD28/CTLA-4 family have been shown to either promote T cell activation (CD28 and ICOS) or downregulate T cell activation (CTLA-4 and PD-1). CD279 is expressed on activated T cells, B cells, myeloid cells and on a subset of thymocytes. In vitro, ligation of CD279 inhibits TCR-mediated T cell proliferation and production of IL-1, IL-4, IL-10 and IFN-γ. In addition, CD279 ligation also inhibits BCR mediated signaling. CD279 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases.

Product References
  1. Antibody-mediated signalling through PD-1 costimulates T cells and enhances CD28- dependent proliferation: M.L. del Rio, et al.; Eur. J. Immunol. 35, 3545 (2005)
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