Chimerigen

CD279 [PD-1] (human):Fc (human) (rec.) (non-lytic)

CHF 520.00

In stock

CHI-HF-220PD1-C200200 µgCHF 520.00

More Information
Product Details
Synonyms	PD-1; Programmed Cell Death Protein 1
Product Type	Protein
Properties
Source/Host	CHO cells
Sequence	The extracellular domain of human CD279 [PD-1] (aa 25-167) is fused to the N-terminus of the Fc region of a mutant human IgG1.
Crossreactivity	Human
Biological Activity	Shows the biological function of the CD279 moiety and exerts a prolonged circulating half-life caused by the modified Fc domain.
Purity	≥98% (SDS-PAGE)
Endotoxin Content	<0.06EU/μg protein (LAL test; Lonza).
Reconstitution	Reconstitute at 100μg/ml in sterile PBS.
Formulation	Lyophilized from 0.2μm-filtered solution in PBS.
Protein Negative Control	Fc (human) IgG1 Control (rec.) (non-lytic)
Other Product Data	Non-lytic: Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC). NCBI reference NP_005009.2: CD279 (human)
Declaration	Manufactured by Chimerigen.
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS	Inquire
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

CD279 (Programmed Cell Death Protein 1; PD-1) is a type I transmembrane protein belonging to the CD28/CTLA-4 family of immunoreceptors that mediate signals for regulating immune responses. Members of the CD28/CTLA-4 family have been shown to either promote T cell activation (CD28 and ICOS) or downregulate T cell activation (CTLA-4 and PD-1). CD279 is expressed on activated T cells, B cells, myeloid cells and on a subset of thymocytes. In vitro, ligation of CD279 inhibits TCR-mediated T cell proliferation and production of IL-1, IL-4, IL-10 and IFN-γ. In addition, CD279 ligation also inhibits BCR mediated signaling. CD279 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases.

Call	+41 61 926 6040
Fax	+41 61 926 6049