Fc (human) IgG1 Control (rec.) (non-lytic)
|Source/Host||HEK 293 cells|
Human IgG Fc region (aa 100-330) containing the hinge sequence.
|Endotoxin Content||<1EU/mg purified protein (LAL test; Lonza).|
|Reconstitution||Reconstitute with 100 µl sterile water. Add 1X PBS to the desired protein concentration.|
|Formulation||Lyophilized from 0.2μm-filtered solution in PBS.|
|Other Product Data||
Non-lytic: Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
|Product Specification Sheet|
This product should be used as the control protein for activity tests of fusion proteins including human target proteins and human IgG-Fc domains.