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AdipoGen Life Sciences
anti-PLXDC2, mAb (Bonny-1) (ATTO 647N)
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Figure 1: Detection of endogenous mouse PLXDC2 with anti-PLXDC2, mAb (Bonny-1) (ATTO 647N) (Prod. No. AG-20B-0086TS).
Method: 3x10^6 thymocytes from WT or Plxdc2 -/- mice are blocked with FcR Mouse Blocker for 10 min at RT, then stained with anti-PLXDC2, mAb (Bonny-1) (ATTO 647N) (1/200) in 100µl of FACS buffer (PBS, 2% FCS, P/S, 1mM EDTA) for 20 min at 4°C. The cells are then stained for surface markers following a standard protocol. Graphs shows DN2 population (CD25+ CD44+) gated from the CD4- CD8- thymocytes. MFI: Mean Fluorescence Intensity. (Picture courtesy of Laurie Bonneaux, Prof. Werner Held Lab, University of Lausanne)
Method: 3x10^6 thymocytes from WT or Plxdc2 -/- mice are blocked with FcR Mouse Blocker for 10 min at RT, then stained with anti-PLXDC2, mAb (Bonny-1) (ATTO 647N) (1/200) in 100µl of FACS buffer (PBS, 2% FCS, P/S, 1mM EDTA) for 20 min at 4°C. The cells are then stained for surface markers following a standard protocol. Graphs shows DN2 population (CD25+ CD44+) gated from the CD4- CD8- thymocytes. MFI: Mean Fluorescence Intensity. (Picture courtesy of Laurie Bonneaux, Prof. Werner Held Lab, University of Lausanne)
| Product Details | |
|---|---|
| Synonyms | Plexin Domain-containing Protein 2; Tumor Endothelial Marker 7-related Protein |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | Bonny-1 |
| Isotype | Mouse IgG2aκ |
| Source/Host | Purified from concentrated hybridoma tissue culture supernatant. |
| Immunogen/Antigen | Recombinant mouse PLXDC2. |
| Label/Conjugates | ATTO 647N |
| Application |
Flow Cytometry: (1:200)
|
| Crossreactivity |
Human Mouse |
| Specificity |
Recognizes endogenous mouse and human PLXDC2. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 0.02% sodium azide. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
Do not freeze. Protect from light. |
| Use/Stability |
Keep conjugated formats at +4°C. Stable for at least 1 year after receipt when stored at +4°C. |
| Documents | |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Plexin domain containing 2 (PLXDC2) is a nidogen/plexin homology transmembrane protein encoded by PLXDC2. PLXDC2 is specifically expressed on the surface of macrophages but not neutrophils, T cells or NK cells. PLXDC2 has several functions: i) in the brain, it is involved in the proliferation and differentiation of neural progenitors during the development of the nervous system; ii) in the immune system, PLXDC2 on macrophages binds to secreted PTEN (phosphatase and tensin homolog) to sequentially activate JAK2-STAT1 signaling, reprogram macrophages and drive antitumor immunity. Aberrant expression of PLXDC2 in different cancers has been reported. PLXDC2, together with its homolog PLXDC1, also seems to be the receptor for pigment epithelium-derived factor (PEDF) in endothelial cells.
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