AdipoGen Life Sciences

anti-Periostin, mAb (blocking) (OC-20) (preservative free)

CHF 470.00
In stock
AG-20B-6000YPF-C100100 µgCHF 470.00
AG-20B-6000YPF-C500500 µgCHF 1'900.00

Replaces AG-20B-6000PF

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Product Details
Synonyms Osteoblast-specific Factor 2; OSF-2; Postn
Product Type Monoclonal Antibody
Clone OC-20
Isotype Mouse IgMκ
Source/Host Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen Recombinant fragments of human periostin.

Functional Application: Blocks interaction with the integrins αvβ3 and αvβ5. Inhibits angiogenesis and tumor growth and blocks allergen-induced inflammation in vivo in mice. It is active at 2µg/ml in vitro and injected at 200µg/mouse for in vivo studies.
Immunocytochemistry: (5μg/ml)
For WB, IHC or ELISA use: anti-Periostin, mAb (Stiny-1) (AG-20B-0033)

Crossreactivity Human

Recognizes human and mouse periostin. Detects the FAS1-2 domain of periostin at a new binding site for the integrins αvβ3 and αvβ5.

Purity ≥95% (SDS-PAGE)
Purity Detail Protein L-affinity purified.
Concentration 1mg/ml
Formulation Liquid. In PBS.
Isotype Negative Control

Mouse IgM Isotype Control

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Periostin is a 90-kDa matricellular protein that consists of a typical signal sequence, followed by a cysteine-rich region, an EMI domain (protein-protein interactions), four tandem fasciclin-like domains that are responsible for integrin binding, and a C-terminal region. Periostin was originally isolated as an osteoblast-specific factor that functions as a cell adhesion molecule for pre-osteoblasts and in osteoblast recruitment, attachment and spreading. Periostin is also involved in many fundamental biological processes such as cell proliferation, cell invasion and angiogenesis. Periostin expression is increased by both transforming growth factor β1 (TGF-β1) and bone morphogenetic protein 2 (BMP-2). Changes in periostin expression are commonly detected in various cancers and pre-cancerous conditions, and periostin may be involved in regulating cancer cell activities that contribute to tumorigenesis, cancer progression and metastasis. Periostin has shown to be involved in many aspects of allergic inflammation, such as eosinophil recruitment, airway remodeling, development of a Th2 phenotype and increased expression of inflammatory mediators. It is evaluated as a biomarker for bronchial asthma and airway inflammation.

Product References
  1. Identification of a novel cell binding site of periostin involved in tumour growth: P. Orecchia, et al.; Eur. J. Cancer 47, 2221 (2011)
  2. Periostin promotes fibrosis and predicts progression in patients with idiopathic pulmonary fibrosis: P.K. Naik, et al.; Am. J. Physiol. Lung Cell Mol. Physiol. 303, L1046 (2012)
  3. Periostin is required for maximal airways inflammation and hyperresponsiveness in mice: J.K. Bentley, et al.; J. Allergy Clin. Immunol. 134, 1433 (2014)
  4. Periostin is a novel therapeutic target that predicts and regulates glioma malignancy: A.M. Mikheev, et al.; Neuro Oncol. 17, 372 (2015)
  5. Periostin: its role in asthma and its potential as a diagnostic or therapeutic target: W. Li, et al.; Resp. Res. 16, 57 (2015)
  6. Imaging of secreted extracellular periostin, an important marker of invasion in the tumor microenvironment in esophageal cancer: P. Heidari, et al.; J. Nucl. Med. 56, 1246 (2015)
  7. Single-cell RNA-seq reveals fibroblast heterogeneity and increased mesenchymal fibroblasts in human fibrotic skin diseases: Ch.Ch. Deng, et al.; Nat. Comm. 12, 3709 (2021)
  8. Periostin secreted by activated fibroblasts in idiopathic pulmonary fibrosis promotes tumorigenesis of non-small cell lung cancer: H. Yamato, et al.; Nat. Sci. Rep. 11, 21114 (2021)
  9. Periostin in lymph node pre‑metastatic niches governs lymphatic endothelial cell functions and metastatic colonization: L. Gillot, et al.; Cell Mol. Life Sci. 79, 295 (2022)
  10. Periostin activates distinct modules of inflammation and itching downstream of the type 2 inflammation pathway: S. Nunomura, et al.; Cell Rep. 42, 111933 (2023)
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