anti-Periostin, mAb (blocking) (OC-20) (preservative free)
|Synonyms||Osteoblast-specific Factor 2; OSF-2; Postn|
|Product Type||Monoclonal Antibody|
|Source/Host||Purified from concentrated hybridoma tissue culture supernatant.|
|Immunogen/Antigen||Recombinant fragments of human periostin.|
Functional Application: Blocks interaction with the integrins αvβ3 and αvβ5. Inhibits angiogenesis and tumor growth and blocks allergen-induced inflammation in vivo in mice. It is active at 2µg/ml in vitro and injected at 200µg/mouse for in vivo studies.
Recognizes human and mouse periostin. Detects the FAS1-2 domain of periostin at a new binding site for the integrins αvβ3 and αvβ5.
|Purity Detail||Protein L-affinity purified.|
|Formulation||Liquid. In PBS.|
|Isotype Negative Control|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
|Use/Stability||Stable for at least 1 year after receipt when stored at -20°C.|
|Product Specification Sheet|
Periostin is a 90-kDa matricellular protein that consists of a typical signal sequence, followed by a cysteine-rich region, an EMI domain (protein-protein interactions), four tandem fasciclin-like domains that are responsible for integrin binding, and a C-terminal region. Periostin was originally isolated as an osteoblast-specific factor that functions as a cell adhesion molecule for pre-osteoblasts and in osteoblast recruitment, attachment and spreading. Periostin is also involved in many fundamental biological processes such as cell proliferation, cell invasion and angiogenesis. Periostin expression is increased by both transforming growth factor β1 (TGF-β1) and bone morphogenetic protein 2 (BMP-2). Changes in periostin expression are commonly detected in various cancers and pre-cancerous conditions, and periostin may be involved in regulating cancer cell activities that contribute to tumorigenesis, cancer progression and metastasis. Periostin has shown to be involved in many aspects of allergic inflammation, such as eosinophil recruitment, airway remodeling, development of a Th2 phenotype and increased expression of inflammatory mediators. It is evaluated as a biomarker for bronchial asthma and airway inflammation.
- Identification of a novel cell binding site of periostin involved in tumour growth: P. Orecchia, et al.; Eur. J. Cancer 47, 2221 (2011)
- Periostin promotes fibrosis and predicts progression in patients with idiopathic pulmonary fibrosis: P.K. Naik, et al.; Am. J. Physiol. Lung Cell Mol. Physiol. 303, L1046 (2012)
- Periostin is required for maximal airways inflammation and hyperresponsiveness in mice: J.K. Bentley, et al.; J. Allergy Clin. Immunol. 134, 1433 (2014)
- Periostin is a novel therapeutic target that predicts and regulates glioma malignancy: A.M. Mikheev, et al.; Neuro Oncol. 17, 372 (2015)
- Periostin: its role in asthma and its potential as a diagnostic or therapeutic target: W. Li, et al.; Resp. Res. 16, 57 (2015)
- Imaging of secreted extracellular periostin, an important marker of invasion in the tumor microenvironment in esophageal cancer: P. Heidari, et al.; J. Nucl. Med. 56, 1246 (2015)
- Single-cell RNA-seq reveals fibroblast heterogeneity and increased mesenchymal fibroblasts in human fibrotic skin diseases: Ch.Ch. Deng, et al.; Nat. Comm. 12, 3709 (2021)
- Periostin secreted by activated fibroblasts in idiopathic pulmonary fibrosis promotes tumorigenesis of non-small cell lung cancer: H. Yamato, et al.; Nat. Sci. Rep. 11, 21114 (2021)
- Periostin in lymph node pre‑metastatic niches governs lymphatic endothelial cell functions and metastatic colonization: L. Gillot, et al.; Cell Mol. Life Sci. 79, 295 (2022)
- Periostin activates distinct modules of inflammation and itching downstream of the type 2 inflammation pathway: S. Nunomura, et al.; Cell Rep. 42, 111933 (2023)