AdipoGen Life Sciences

anti-PARP-10 [ARTD10] (human), mAb (5H11)

CHF 380.00

In stock

AG-20T-0004-L0011 mlCHF 380.00

Figure 1: Immunofluorescence using anti-PARP-10 [ARTD10] (human), mAb (5H11). Method: T47D mammary carcinoma cells were stained by indirect immunofluorescence and analysed. Prior to fixation and staining, the cells were treated with or without 50nm leptomycin B (LMB) as indicated for 2 h. Controls identify samples that were not incubated with the primary antibody (mAb 5H11) but only with the secondary antibody. The exposure times were identical for the stainings with and without primary antibody. DNA was identified by Hoechst stain. A phase contrast picture of the labeled cells is shown as well.

Specifications / Handling

More Information
Product Details
Synonyms	ADP-ribosyltransferase Diphtheria Toxin-like 10; Poly(ADP-ribose) Polymerase-10
Product Type	Monoclonal Antibody
Properties
Clone	5H11
Isotype	Rat IgG1
Immunogen/Antigen	Recombinant human PARP-10 [ARTD10] (aa 1-907) fused to a GST-tag.
Application	Immunocytochemistry:(1:50-1:200) Immunoprecipitation:(1:10) Western Blot:(1:50) Optimal conditions must be determined individually for each application.
Crossreactivity	Human
Specificity	Recognizes human PARP-10 [ARTD10] with an epitope between aa 256-407, containing the glycine rich (G-rich) region.
Endotoxin Content
Formulation	Liquid. Cell culture supernatant containing RPMI, 10% FCS and 0.05% sodium azide.
Isotype Negative Control	Rat IgG1 Isotype Control
Other Product Data	http://www.uniprot.org/uniprot/Q53GL7
Accession Number	Q53GL7
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	+4°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 1 year after receipt when stored at +4°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

PARP-10 [ARTD10] is a mono-ADP-ribosyltransferase and was initially identified as an interaction partner of the proto-oncoprotein Myc. PARP-10 possesses ubiquitin-interaction motifs, which are important to inhibit the activation of NF-κB and downstream target genes in response to interleukin-1β [IL-1β] and tumor necrosis factor-α [TNF-α]. PARP-10 mono(ADP-ribosyl)ates (MARylates) NEMO (NF-κB essential modulator), which results in reduced NEMO polyubiquitylation and thus decreased NF-κB signaling. Overexpression of PARP-10 was shown to lead to apoptosis. PARP-10 knockdown leads to increased cell survival. Additional substrates of PARP-10, such as GSK-3β were identified and PARP-10-mediated mono(ADP-ribosyl)ation (MARylation) was shown to be involved in regulating multiple processes, including Wnt signaling.

Product References

PARP-10, a novel Myc-interacting protein with poly(ADP-ribose) polymerase activity, inhibits transformation: M. Yu, et al.; Oncogene 24, 1982 (2005)
Dynamic subcellular localization of the mono-ADP-ribosyltransferase ARTD10 and interaction with the ubiquitin receptor p62: H. Kleine, et al.; Cell Commun. Signal. 10, 28 (2012)
Regulation of NF-κB signalling by the mono-ADP-ribosyltransferase ARTD10: P. Verheugd, et al.; Nat. Commun. 4, 1683 (2013)
ARTD10 substrate identification on protein microarrays: regulation of GSK3β by mono-ADP-ribosylation: K.L. Feijs, et al.; Cell Commun. Signal. 11, 1 (2013)
Macrodomain-containing proteins: regulating new intracellular functions of mono(ADP-ribosyl)ation: K.L. Feijs, et al.; Nat. Rev. Mol. Cell Biol. 13, 443 (2013)
Caspase-dependent cleavage of the mono-ADP-ribosyltransferase ARTD10 interferes with its pro-apoptotic function: N. Herzog, et al.; FEBS J. 280, 1330 (2013)

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