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AdipoGen Life Sciences
anti-Caspase-12 (mouse), mAb (12G6)
350
CHF
CHF 350.00
In stock
AG-20T-0141-C100100 µgCHF 350.00
Figure 1: Western blot analysis using anti-caspase-12 (mouse), mAb (12G6) (Prod. No. AG-20T-0141) detecting, A) endogenous caspase-12 in mouse lung, liver, spleen and pancreas, and B) pro-caspase-12 in mouse MEF’s.
| Product Details | |
|---|---|
| Synonyms | CASP-12 |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | 12G6 |
| Isotype | Rat IgG1κ |
| Immunogen/Antigen | Recombinant mouse caspase-12 (p20 fragment) and synthetic peptide corresponding to aa 183-205 of mouse caspase-12. |
| Application |
ELISA |
| Crossreactivity | Mouse |
| Specificity |
Recognizes an epitope within the p20 fragment of mouse caspase-12. Detects a band of ~51kDa by Western blot. Does not cross-react with mouse caspase-1 or -11. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified (sepharose column). |
| Endotoxin Content | <0.1EU/μg purified protein (LAL test; Lonza). |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 0.02% sodium azide. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Caspase-12 is a member of the ICE/CED-3 family of caspases and is detected as a protein of ~51kDa. Overexpression of caspase-12 in cells induces apoptosis. Capase-12 is localized to the endoplasmatic reticulum (ER) and activated by ER stress and specifically cleaves and activates pro-caspase-9 and is also thought to be involved in the apoptosis induced by viral infection. Caspase-12-deficient mice are resistant to ER stress-induced apoptosis. Caspase-12 is probably expressed in many and perhaps all mouse tissues and is expressed at high levels in skeletal muscle, heart, lung and kidney and at moderate levels in brain and liver. In many humans caspase-12 is a pseudo gene and not expressed.