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AdipoGen Life Sciences
anti-BimS/EL/L, mAb (3C5)
420
CHF
CHF 420.00
In stock
AG-20T-0142-C100100 µgCHF 420.00
Figure 1: Flow cytometry data in permeabilized and fixed transfected cells using anti-Bim, mAb (3C5) (Prod. No. AG-20T-0142), anti-EE (positive control) or secondary antibody alone (negative control). Staining was visualised by FITC-conjugated goat anti-rat or anti-mouse IgG antibodies.
Figure 2: A) Western blot using anti-Bim, mAb (3C5) (Prod. No. AG-20T-0142) detecting EE mouse BimS, BimL, BimEL and EE human, BimSS, BimS, BimLS, BimL, BimELS, BimEL. Probing with an anti-Hsp-70 mAb was used as a loading control for all blots. B) Western blot using anti-Bim, mAb (3C5) detecting endogenous Bim expression in mouse tissues but not in Bim knockout mice. Bim protein was revealed in lysates from mouse tissues (5 x 106/lane) and detection by ECL. Probing with an anti-Hsp-70 mAb was used as a loading control.
| Product Details | |
|---|---|
| Synonyms | BCL2L11; Bcl-2-like Protein 11; Bcl2-interacting Mediator of Cell Death |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | 3C5 |
| Isotype | Rat IgG2aκ |
| Immunogen/Antigen | Synthetic peptide corresponding to aa 20-40 of mouse BimL. |
| Application |
ELISA |
| Crossreactivity |
Dog Human Monkey Mouse Rat |
| Specificity |
Recognizes an epitope within aa 20-40 of human, mouse, rat, monkey and dog BimL (~19kDa), BimEL (~23kDa), and BimS (~16kDa). Also recognizes the six novel isoforms of human Bim, generated by alternative splicing and lacking the C-terminal hydrophobic region. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified (sepharose column). |
| Endotoxin Content | <0.1EU/μg purified protein (LAL test; Lonza). |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 0.02% sodium azide and 25% glycerol. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Bim belongs to the Bcl-2 protein family. Bcl-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the Bcl-2 protein family (BCL2, BCL2L1/BCL-X(L) and MCL1) and to act as an apoptotic activator, inducing apoptosis and anoikis. Several variants have been identified. The isoforms vary in cytotoxicity with isoform BimS being the most potent and isoform BimEL being the least potent.
Product References
- Generalized resistance to thymic deletion in the NOD mouse; a polygenic trait characterized by defective induction of Bim: A. Liston, et al.; Immunity 21, 817 (2004)
- Bim is a suppressor of Myc-induced mouse B cell leukemia: A. Eagle, et al.; PNAS 101, 6164 (2004)
- ER stress triggers apoptosis by activating BH3-only protein Bim: H. Puthalakath, et al.; Cell 129, 1337 (2007)
- BimS-induced apoptosis requires mitochondrial localization but not interaction with anti-apoptotic Bcl-2 proteins: A. Weber, et al.; J. Cell Biol. 177, 625 (2007)
- NF-κB1 and c-Rel cooperate to promote the survival of TLR4-activated B cells by neutralizing Bim via distinct mechanisms: A. Banerjee, et al.; Blood 112, 5063 (2008)
- Loss of the BH3-only protein Bmf impairs B cell homeostasis and accelerates γ irradiation-induced thymic lymphoma development: V. Labi, et al.; J. Exp. Med. 205, 641 (2008)
- MEK/ERK-Mediated Phosphorylation of Bim is Required to Ensure Survival of T and B Lymphocytes during Mitogenic Stimulation: L.A. O'Reilly, et al.; J. Immunol. 183, 261 (2009)
- c-Cbl promotes T cell receptor-induced thymocyte apoptosis by activating the phosphatidylinositol 3-kinase/Akt pathway: C.B. Thien, et al.; J. Biol. Chem. 285, 10969 (2010)
- Targeting Activating Transcription Factor 3 by Galectin-9 Induces Apoptosis and Overcomes Various Types of Treatment Resistance in Chronic Myelogenous Leukemia: J. Kuroda, et al.; Mol. Cancer Res. 8, 994 (2010)
- OCK8 deficiency impairs CD8 T cell survival and function in humans and mice: K.L. Randall, et al.; J. Exp. Med. 208, 2305 (2011)
- Cyclic-AMP-dependent protein kinase A regulates apoptosis by stabilizing the BH3-only protein Bim: D. Moujalled, et al.; EMBO Rep. 12, 77 (2011)
- Alternative splicing of Bim and Erk-mediated Bim(EL) phosphorylation are dispensable for hematopoietic homeostasis in vivo: C. Clybouw, et al.; Cell Death Differ. 19, 1060 (2012)
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