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AdipoGen Life Sciences
anti-β-Pix (mouse), pAb
190
CHF
CHF 190.00
In stock
AG-25A-0006-C100100 µgCHF 190.00
Figure 1: Western blot analysis using anti-β-Pix (mouse), pAb (Prod. No. AG-25A-0006).
1: Mouse brain lysates (30μg).
2: NIH3T3 whole cell lysates (30μg).
* β-Pix is a major isoform in NIH3T3 lysates, while other β-Pix isoforms are also detected in mouse brain lysates.
1: Mouse brain lysates (30μg).
2: NIH3T3 whole cell lysates (30μg).
* β-Pix is a major isoform in NIH3T3 lysates, while other β-Pix isoforms are also detected in mouse brain lysates.
| Product Details | |
|---|---|
| Synonyms | ARHGEF7; Rho Guanine Nucleotide Exchange Factor 7; PAK-interacting Exchange Factor β; p85SPR; COOL-1 |
| Product Type | Polyclonal Antibody |
| Properties | |
| Source/Host | Rabbit |
| Immunogen/Antigen | Recombinant β-Pix SH3 domain. |
| Application |
ELISA: (direct and indirect: 1:5'000-1:10'000) Note: Tested on recombinant proteins and/or target-protein transfected cell lines in ELISA, Western Blot and/or FACS. |
| Crossreactivity |
Human Mouse Rat |
| Specificity |
Recognizes the multiple splice variants of human, mouse and rat β-Pix a, b, bL, c and d-isoforms. Cross-reacts with human and rat β-Pix. |
| Purity Detail | Protein A-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 6 months after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
β-PIX acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. It interacts with PAK kinases through the SH3 domain. It may function as a positive regulator of apoptosis and is involved in cell migration. It is expressed with high levels in the central nervous system.
Product References
- Cloning of a SH3 domain-containing proline-rich protein, p85SPR, and its localization in focal adhesion: W.K. Oh, et al.; BBRC 235, 794 (1997)
- betaPix-b(L), a novel isoform of betaPix, is generated by alternative translation: S. Rhee, et al.; BBRC 318, 415 (2004)
- Enhanced cytotoxic effect of camptothecin nanosponges in anaplastic thyroid cancer cells in vitro and in vivo on orthotopic xenograft tumors: C.L. Gigliotti, et al.; Drug Deliv. 24, 670 (2017)
- Differential Modulation of Human M1 and M2 Macrophage Activity by ICOS‐Mediated ICOSL Triggering: C.L. Gigliotti, et al.; Int. J. Mol. Sci. 24, 2953 (2023)