AdipoGen Life Sciences

anti-Leptin (rat), pAb

CHF 390.00

In stock

AG-25A-0009-C100100 µgCHF 390.00

Figure 1: Western blot analysis using anti-Leptin (rat), pAb (Prod. No. AG-25A-0009) at 1:5'000 dilution.

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Product Details
Synonyms	OB Gene Product; Obese Factor
Product Type	Polyclonal Antibody
Properties
Source/Host	Rabbit
Immunogen/Antigen	Recombinant rat leptin.
Application	ELISA: (direct and indirect: 1:5’000-1:10’000) Western Blot: (1:5’000-1:10’000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
Crossreactivity	Rat
Specificity	Recognizes rat leptin. Weakly cross-reacts with human and mouse leptin. Detects a band of ~16kDa by Western blot.
Purity Detail	Protein A-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Rabbit IgG
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Leptin is involved in the signaling pathway that regulates the size of the body fat depot. An increase in the level of leptin may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in the leptin gene may be a cause of autosomal recessive obesity.

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