AdipoGen Life Sciences

anti-Resistin (human), pAb

CHF 390.00

In stock

AG-25A-0013-C100100 µgCHF 390.00

Figure 1: Western blot analysis of adipocyte-conditioned medium from human male and female with different expression levels using anti-Resistin (human), pAb (Prod. No. AG-25A-0013).

Figure 2: Immunoblot analysis of adipocyte-conditioned medium from human male and female with different Resistin expression levels using anti-Resistin (human), pAb (Prod. No. AG-25A-0013). The molecular weight of Resistin is ~10kDa.

Specifications / Handling

More Information
Product Details
Synonyms	FIZZ3; Found in Inflammatory Zone 3; ADSF; Adipose Tissue-Specific Secretory Factor
Product Type	Polyclonal Antibody
Properties
Source/Host	Rabbit
Immunogen/Antigen	Recombinant human resistin.
Application	ELISA: (direct and indirect: 1:5’000-1:10’000) Western Blot: (1:2’000-1:10’000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
Crossreactivity	Human
Specificity	Recognizes human resistin. Weakly cross-reacts with mouse resistin. Detects a band of ~10kDa by Western blot.
Purity Detail	Protein A-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Rabbit IgG
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

The adipocytokine resistin which belongs to a family of cysteine-rich C-terminal proteins known as resistin-like molecules (RELM; RELMα/FIZZ 1 and RELMβ/FIZZ 2) of FIZZ (found in inflammatory zone) are thought to be involved in inflammatory processes. Previous studies in mice showed that resistin impairs glucose tolerance and insulin action. In addition, resistin also inhibits adipogenesis in murine 3T3-L1 cells. Therefore resistin has also been proposed as an adipocyte-secreted factor thought to link obesity and T2DM.

Product References

Common genetic polymorphisms in the promoter of resistin gene are major determinants of plasma resistin concentrations in humans: Y.M. Cho, et al.; Diabetologia 47, 559 (2004)
Plasma resistin concentrations measured by enzyme-linked immunosorbent assay using a newly developed monoclonal antibody are elevated in individuals with type 2 diabetes mellitus: B.S. Youn, et al.; J. Clin. Endocrinol. Metab. 89, 150 (2004)
Differential impact of adipokines derived from primary adipocytes of wild-type versus streptozotocin-induced diabetic rats on glucose and fatty acid metabolism in cardiomyocytes: R. Palanivel, et al.; J. Endocrinol. 199, 389 (2008)

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