anti-Resistin (human), pAb (Biotin)
AG-25A-0013B-C05050 µgCHF 390.00
Western blot analysis of adipocyte-conditioned medium from human male and female with different expression levels using anti-Resistin (human), pAb (Biotin) (Prod. No. AG-25A-0013B).
|Synonyms||FIZZ3; Found in Inflammatory Zone 3; ADSF; Adipose Tissue-Specific Secretory Factor|
|Product Type||Polyclonal Antibody|
|Immunogen/Antigen||Recombinant human resistin.|
ELISA: (direct and indirect: 1:5’000-1:10’000)
Western Blot: (1:2’000-1:10’000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
|Specificity||Recognizes human resistin. Weakly cross-reacts with mouse resistin. Detects a band of ~10kDa by Western blot.|
|Purity Detail||Protein A-affinity purified.|
|Formulation||Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
|Use/Stability||Stable for at least 6 months after receipt when stored at -20°C.|
|Product Specification Sheet|
The adipocytokine resistin which belongs to a family of cysteine-rich C-terminal proteins known as resistin-like molecules (RELM; RELMα/FIZZ 1 and RELMβ/FIZZ 2) of FIZZ (found in inflammatory zone) are thought to be involved in inflammatory processes. Previous studies in mice showed that resistin impairs glucose tolerance and insulin action. In addition, resistin also inhibits adipogenesis in murine 3T3-L1 cells. Therefore resistin has also been proposed as an adipocyte-secreted factor thought to link obesity and T2DM.
- Common genetic polymorphisms in the promoter of resistin gene are major determinants of plasma resistin concentrations in humans: Y.M. Cho, et al.; Diabetologia 47, 559 (2004)
- Plasma resistin concentrations measured by enzyme-linked immunosorbent assay using a newly developed monoclonal antibody are elevated in individuals with type 2 diabetes mellitus: B.S. Youn, et al.; J. Clin. Endocrinol. Metab. 89, 150 (2004)
- Differential impact of adipokines derived from primary adipocytes of wild-type versus streptozotocin-induced diabetic rats on glucose and fatty acid metabolism in cardiomyocytes: R. Palanivel, et al.; J. Endocrinol. 199, 389 (2008)