AdipoGen Life Sciences

anti-RBP4 (mouse), pAb

CHF 390.00

In stock

AG-25A-0036-C100100 µgCHF 390.00

Figure 1: Western blot analysis using anti-RBP4 (mouse), pAb (Prod. No. AG-25A-0036) at 1:2'000 dilution.
1: Mouse RBP4 (His-tagged).
2: Mouse serum (ob/ob) (2μl).

Specifications / Handling

More Information
Product Details
Synonyms	Retinol Binding Protein 4
Product Type	Polyclonal Antibody
Properties
Source/Host	Rabbit
Immunogen/Antigen	Recombinant mouse RBP4.
Application	ELISA: (direct and indirect: 1:2’000-1:5’000) Western Blot: (1:2’000-1:5’000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
Crossreactivity	Mouse
Specificity	Recognizes mouse RBP4. Detects a band of ~22-25kDa by Western blot.
Purity Detail	Protein A-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Rabbit IgG
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Retinol binding protein 4 (RBP4; RBP) is a 21kDa secreted protein, a member of the lipocalin family and is known as the primary transporter of retinol (vitamin A) to tissues. A recent report revealed RBP4 as an adipokine linking glucose transporter 4 (GLUT4) suppression in adipose tissue to insulin. Elevated human and mouse serum RBP4 levels are associated with insulin resistance and its severity, obesity and certain components of metabolic syndrome. Furthermore, human serum RBP4 levels are closely related to renal function.

Product References

The cAMP-HMGA1-RBP4 system: a novel biochemical pathway for modulating glucose homeostasis: E. Chiefari, et al.; BMC Biol. 7, 24 (2009)
Interleukin-6 mediates exercise-induced increase in insulin sensitivity in mice: A. Benrick, et al.; Exp. Physiol. 97, 1224 (2012)

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